Summary of Study ST003251
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002018. The data can be accessed directly via it's Project DOI: 10.21228/M8RV57 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003251 |
| Study Title | Temperature change elicits lipidome adaptation in the simple organisms Mycoplasma mycoides and JCVI-syn3B |
| Study Summary | Cell membranes mediate interactions between life and its environment, with lipids determining their properties. Understanding how cells adjust their lipidomes to tune membrane properties is crucial yet poorly defined due to the complexity of most organisms. We used quantitative shotgun lipidomics to study temperature adaptation in the simple organism Mycoplasma mycoides and the minimal cell, JCVI-syn3B. We show that lipid abundances follow a universal logarithmic distribution across eukaryotes and bacteria, with comparable degrees of lipid remodeling for adaptation regardless of lipidomic or organismal complexity. Lipid features analysis demonstrates head group-specific acyl chain remodeling as characteristic of lipidome adaptation; its deficiency in Syn3B is associated with impaired homeoviscous adaptation. Temporal analysis reveals a two-stage cold adaptation process: swift cholesterol and cardiolipin shifts followed by gradual acyl chain modifications. This work provides an in-depth analysis of lipidome adaptation in minimal cells, laying a foundation to probe the design principles of living membranes. |
| Institute | TU Dresden |
| Last Name | Saenz |
| First Name | James |
| Address | Tatzberg 41, Dresden, Saxony, 01307, Germany |
| jpsaenz81@gmail.com | |
| Phone | (+49) 351 463 43066 |
| Submit Date | 2024-05-30 |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003363 |
| Collection Summary: | Lipidomics data of M. mycoides and JCVI-syn3B membranes,extracted at different temperatures and time points, represented as molp for each of the samples. Quantification in mol % sample. mol% data were calculated, using pmols of lipid intensities (calculated from intensities of each lipid, measured m/z from the mass spectrometry), standardised to total lipid amount. |
| Collection Protocol Filename: | 240528_TempAdapt_Manuscript_Data.xlsx |
| Sample Type: | Mycoplasma |
