Summary of Study ST003282
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002034. The data can be accessed directly via it's Project DOI: 10.21228/M8PV5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003282 |
| Study Title | 96-plex metabolomics studies on nutrient-deprived endothelial cells |
| Study Summary | A NeuCode tag which allows for cost efficient incorporation of isotopes is used to enable a 96-plex assay for small molecule metabolomics. A 96-well plate of cell lysates is analyzed simultaneously, providing absolute quantitation of 38 metabolites across 96 samples in a single LC-MS injection. |
| Institute | Saint Louis University |
| Last Name | Armbruster |
| First Name | Michael |
| Address | 4448 Swiss Stone Ln E Apt 3C, Ypsilanti, Michigan, 48197, USA |
| armbrustermr@gmail.com | |
| Phone | 3145501620 |
| Submit Date | 2024-02-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003395 |
| Collection Summary: | Cell culture media was removed and the cells were rinsed 3x with PBS using an 8-channel pipette. Lysing solution (100µL of 80/20 ACN/H2O)) was added and the cells were put over ice. The wells were flush-mixed 10 times, sonicated for 5 minutes, then centrifuged at 3,400 rpm for 10 minutes at 10ºC. The supernatant was then transferred to a 96-well plate containing 200µg of pre-aliquoted tag in each well. |
| Sample Type: | Endothelial cells |
