Summary of Study ST000446
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000344. The data can be accessed directly via it's Project DOI: 10.21228/M8KK69 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000446 |
Study Title | Heterologous expression and detection of Apratoxins in E. coli |
Study Type | Organic extraction of E coli cultures harboring apratoxin gene cluster |
Study Summary | The apratoxin gene cluster was recovered from fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried. |
Institute | University of Florida |
Department | SECIM |
Last Name | Kallifidas |
First Name | Dimitris |
Address | Medical Sciences Building, Rm P5-26, 1600 SW Archer Rd., FL32610 |
dkallifidas@ufl.edu | |
Phone | None |
Submit Date | 2016-07-28 |
Num Groups | 1 |
Total Subjects | 12 |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Collection:
Collection ID: | CO000461 |
Collection Summary: | 50 ml of LB broth inoculated with the appropriate E. coli strains were extracted twice with equal volume of etheyl acetate and dried. |
Sample Type: | Dried ethyl acetate cell extracts |