Summary of Study ST001090
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000729. The data can be accessed directly via it's Project DOI: 10.21228/M8FM38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001090 |
| Study Title | NMR metabolomics study of Gemcitabine resistant cancer cells |
| Study Type | NMR metabolomics |
| Study Summary | 1D 1H NMR spectra were collected from the samples and analyzed using MVAPACK software (http://bionmr.unl.edu/mvapack.php). Multivariate analysis was conducted to study the metabolic phenotypes of the samples. |
| Institute | University of Nebraska-Lincoln |
| Department | Chemistry |
| Laboratory | Dr. Robert Powers and Pankaj Singh labs |
| Last Name | Gebregiworgis |
| First Name | Teklab |
| Address | 552 Hamilton Hall, 639 N. 12th Street, Lincoln, NE, 68588, USA |
| teklab@huskers.unl.edu | |
| Phone | (402) 472-3039 |
| Submit Date | 2018-09-25 |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2019-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001128 |
| Collection Summary: | For each of the cell culture, 1 x 106 cells were cultured and harvested at 70-80% confluence in a 100 mm Petri dish. The media was then removed by aspiration upon harvesting, and the cells were washed twice with PBS at pH 7.2. The cells were then lysed by the addition of 1 mL of an 80% methanol:water mixture at -80oC followed by incubation for 15 minutes in a -80oC freezer. The lysed cells were then transferred to a 1 ml Eppendorf tube using a cell scraper. The cell lysate was centrifuged at 16,200 g for 5 minutes and the supernatant was collected and transferred to a clean Eppendorf tube. 250 µL of nanopure water (Nanopure, Dubuque, IA) was added to the cell debris, the sample was mixed by pipetting, and then centrifuged as before. The two supernatants were combined, and the sample was dried by vacuum evaporation (SpeedVac® Plus, Savant, Thermo Scientific, Waltham, MA) followed by freeze-drying (Labconco, Kansas City, MO). |
| Sample Type: | Cultured cells |
| Storage Conditions: | -80℃ |
