Summary of Study ST001512
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001019. The data can be accessed directly via it's Project DOI: 10.21228/M80408 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001512 |
Study Title | Diel investments in phytoplankton metabolite production influenced by associated heterotrophic bacteria |
Study Type | Incubation experiment |
Study Summary | Organic substrate transfer between photoautotrophic and heterotrophic microbes in the surface ocean is a central but poorly understood process in the global carbon cycle. This study developed a co-culture system of marine diatom Thalassiosira pseudonana and heterotrophic bacterium Ruegeria pomeroyi, and addressed diel changes in phytoplankton endometabolite production using nuclear magnetic resonance (NMR) and bacterial metabolite consumption using gene expression. Here we deposit data for NMR analysis from the study. Samples were collected every 6 hours over two days under a diel light cycle. During the course of the study, we observed an increase in some phytoplankton endometabolites presumably due to the effects of the associated bacteria. We introduced an additional experiment and tested this possibility by comparing phytoplankton endometabolite accumulation between axenic treatments and bacteria coculture treatments. |
Institute | University of Georgia |
Department | Department of Marine Sciences; Complex Carbohydrate Research Center |
Laboratory | Moran Lab; Edison Lab |
Last Name | Uchimiya |
First Name | Mario |
Address | 315 Riverbend Rd, Athens, GA 30602 |
mario.uchimiya@uga.edu | |
Phone | (706) 542-8387 |
Submit Date | 2020-10-18 |
Raw Data Available | Yes |
Analysis Type Detail | NMR |
Release Date | 2020-11-02 |
Release Version | 1 |
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Collection:
Collection ID: | CO001581 |
Collection Summary: | Diel experiment – Samples were collected every 6 h over 2 days. 500 mL of diatom cells were collected by filtering the culture onto 2.0-µm-pore-size PCTE membrane filters (MilliporeSigma Isopore). Filters were kept in 50 mL tubes (Falcon) and stored at -80oC until processing. Test of model prediction experiment – The same procedure was used except that samples were collected at day 15 and 700 mL of the culture was filtered. |
Collection Protocol Filename: | uchimiya_20201018_2_Collection protocol_UGA_phytoplankton_Oct2020.docx |
Collection Protocol Comments: | Collection summary is in 2_Collection protocol_UGA_phytoplankton_Oct2020.docx |
Sample Type: | Algae |