Summary of Study ST001941
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001229. The data can be accessed directly via it's Project DOI: 10.21228/M8V98S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001941 |
| Study Title | Untargeted metabolomics of breast cell lines in the presence or the absence of CtBP inhibitors |
| Study Summary | Experiments to test the effect of CtBP2 inhibition on metabolism of breast cell lines. In particular, experiment 1 involves comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231). Experiment 2 is a study between MDA-MB231 silenced for CtBP2 by stable RNA interference (shCtBP2 cells) compared to scramble (shCTRL cells). Experiment 3 is a comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231)in the presence of the absence of small-molecule CtBP inhibitors: HIPP (400 μM) or P4 (300 μM)for 48 hours. |
| Institute | University of Milano-Bicocca |
| Last Name | Bonanomi |
| First Name | Marcella |
| Address | Piazza della Scienza 4 |
| marcella.bonanomi@unimib.it | |
| Phone | +390264483343 |
| Submit Date | 2021-08-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002012 |
| Collection Summary: | MDA-MB231 and MCF102A cell lines were obtained from the American Type Culture Collection (ATCC) (LGC Standard, UK). MDA-MB231 cell line was grown in Dulbecco's modified Eagle's medium (DMEM) containing 4mM L-glutamine, supplemented with 10% fetal bovine serum. MCF102A cell line was maintained in DMEM/F-12 containing 5% horse serum, 2.5mM L-glutamine, 20ng/ml EGF, 100 ng/ml cholera toxin, 0.01 mg/ml insulin, and 500 ng/ml hydrocortisone. All media were supplemented with 100 U/ml penicillin and 100 µg/ml streptomycin, and cells were incubated at 37°C in a 5% CO2 incubator. |
| Sample Type: | Breast cancer cells |
