Summary of Study ST001987
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001263. The data can be accessed directly via it's Project DOI: 10.21228/M8FX3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001987 |
| Study Title | Transcriptomic and lipidomic analysis unravels the response of Faecalibacterium prausnitzii to calcium palmitate |
| Study Summary | Infant formula is a suggested alternative to human milk if breastfeeding is not an option; vegetable oil blends are commonly used in infant formula (IF) to replace dairy fat, which can induce the formation of the poorly soluble soap calcium palmitate (CP) in the infant’s gut. Previously, we observed that CP at a low concentration of 0.01 mg/ml inhibits the growth of dominant infant bacteria such as Faecalibacterium prausnitzii both during the exponential phase as well as in the stationary phase. Here, we investigate the underlying mechanism of the CP inhibition on infant-gut bacteria using F. prausnitzii as a model by analysing its growth at a transcriptomic and lipidomic level. |
| Institute | University of Groningen |
| Last Name | Horvatovich |
| First Name | Péter |
| Address | Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands |
| p.l.horvatovich@rug.nl | |
| Phone | +31 (0)50 363 3341 |
| Submit Date | 2021-11-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-12-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002061 |
| Collection Summary: | Lipids were extracted using the MTBE method (Gil et al., 2018). The bacterial cell pellet was resuspended into 75 μL water, then it was incubated with 200 μL methanol and 625 μL MTBE (methyl tert-butyl ether) for 1 h on a shaker. The bacteria/methanol/MTBE mixture was further incubated with 100 μL water to induce phase separation. After 10 min centrifugation at 1000 × g, the upper organic phase was collected. The lower phase was re-extracted with 250 μL MTBE/methanol/water 10:3:2.5 v/v/v and incubated on a shaker for 30 min. After 3 min centrifugation at 1000 × g, the upper organic phase was collected. The combined organic phases containing the lipids were dried in a vacuum centrifuge at 30 ºC and then dissolved in 25 μL CHCl3/methanol/water 60:30:4.5 v/v/v. 75 μL isopropanol/acetonitrile/water 2:1:1 v/v/v was added to dilute the lipid solution. |
| Sample Type: | Bacteria |
| Collection Location: | University Medical Center Groningen (UMCG) |
