Summary of Study ST002357
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002357 |
| Study Title | Comparative lipidomics study of brain samples from deep-diving pinnipeds and terrestrial (non-diving) relatives |
| Study Summary | Brain samples from deep-diving pinnipeds (Cystophora cristata (n=10), Pagophilus groenlandicus (n=6)) were compared to terrestrial (non-diving) relatives (Mustela putorius furo (n=4), Mus musculus (n=9)). |
| Institute | University of Hamburg |
| Last Name | Martens |
| First Name | Gerrit Alexander |
| Address | Martin-Luther-King-Platz 3, 20146 Hamburg, Germany |
| gerrit.alexander.martens@uni-hamburg.de | |
| Phone | +49 40 42838-3934 |
| Submit Date | 2022-11-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-12 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002439 |
| Collection Summary: | Hooded seals (Cystophora cristata) and harp seals (Pagophilus groenlandicus) were captured for other scientific purposes in the pack ice of the Greenland Sea under permits from relevant Norwegian and Greenland authorities. The hooded seal pups were brought to UiT – The Arctic University of Norway, where they were maintained in a certified research animal facility in connection with other studies. At the termination of those experiments, the seals were euthanized (at age 1 year, respectively) in accordance with a permit issued by the National Animal Research Authority of Norway (permits no. 7247, 19305 and 22751): the seals were sedated by intramuscular injection of zolazepam/tiletamine (Zoletil Forte Vet., Virbac S.A., France; 1.5–2.0 mg per kg of body mass), then anaesthetized using an endotracheal tube to ventilate lungs with 2–3% isoflurane (Forene, Abbott, Germany) in air and, when fully anaesthetized, they were euthanized by exsanguination via the carotid arteries. The adult hooded and harp seals were euthanized immediately following capture, by sedation with intramuscular injection of zolazepam/tiletamine (1.5–2.0 mg per kg of body mass), followed by catheterization of the extradural intravertebral vein and i.v. injection of an overdose of pentobarbital (Euthasol vet., Le Vet B.V., Netherlands; 30 mg per kg of body mass). All animal handling was in accordance with the Norwegian Animal Welfare Act and with approvals from the National Animal Research Authority of Norway (permits no. 7247, 19305 and 22751). Samples of adult female ferrets were received from the animal facilities of the University Medical Center Hamburg-Eppendorf (UKE) Germany. The ferrets were killed at the UKE in deep anesthesia (Ketamin/Domitor) with an overdose of pentobarbital and brain tissues were sampled by the facility’s veterinarians. Adult female mice (C57BL/6) were a gift by Prof. Dr. Christian Lohr (University of Hamburg, Hamburg, Germany) and were anaesthetized with 1 ml isoflurane (Forene, Abbott, Germany) in a chamber and decapitated. All animals were handled according to the EU Directive 63 (Directive 2010/63/EU). Fresh brain tissue of all animals was frozen in liquid nitrogen and transferred to -80 °C to be stored for subsequent analysis. |
| Sample Type: | Brain |
| Storage Conditions: | -80℃ |
