Summary of Study ST002358
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002358 |
| Study Title | Effect of hypoxia and reoxygenation on the juvenile hooded seal brain lipidome |
| Study Summary | Brain samples from juvenile hooded seals (Cystophora cristata) were subjected to hypoxia and reoxygenation in vitro and lipid composition was compared. |
| Institute | University of Hamburg |
| Last Name | Martens |
| First Name | Gerrit Alexander |
| Address | Martin-Luther-King-Platz 3, 20146 Hamburg, Germany |
| gerrit.alexander.martens@uni-hamburg.de | |
| Phone | +49 40 42838-3934 |
| Submit Date | 2022-11-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-12 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002440 |
| Collection Summary: | Hooded seals (Cystophora cristata) were captured for other scientific purposes in the pack ice of the Greenland Sea under permits from relevant Norwegian and Greenland authorities. The hooded seal pups were brought to UiT – The Arctic University of Norway, where they were maintained in a certified research animal facility in connection with other studies. At the termination of those experiments, the seals were euthanized (at age 1 year, respectively) in accordance with a permit issued by the National Animal Research Authority of Norway (permits no. 7247, 19305 and 22751): the seals were sedated by intramuscular injection of zolazepam/tiletamine (Zoletil Forte Vet., Virbac S.A., France; 1.5–2.0 mg per kg of body mass), then anaesthetized using an endotracheal tube to ventilate lungs with 2–3% isoflurane (Forene, Abbott, Germany) in air and, when fully anaesthetized, they were euthanized by exsanguination via the carotid arteries. All animal handling was in accordance with the Norwegian Animal Welfare Act and with approvals from the National Animal Research Authority of Norway (permits no. 7247, 19305 and 22751). Fresh visual cortex and hippocampus samples from hooded seal pups were minced and placed in cooled (4 °C) artificial cerebrospinal fluid (aCSF; 128 mM NaCl, 3 mM KCl, 1.5 mM CaCl2, 1 mM MgCl2, 24 mM NaHCO3, 0.5 mM NaH2PO4, 20 mM sucrose, 10 mM D-glucose) saturated with 95% O2−5% CO2 (normoxia) and further processed in vitro. |
| Sample Type: | Brain |
| Storage Conditions: | -80℃ |
