Summary of Study ST002359
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002359 |
Study Title | Effect of hypoxia and reoxygenation on the adult hooded seal brain lipidome |
Study Summary | Brain samples from adult hooded seals (Cystophora cristata) were subjected to hypoxia and reoxygenation in vitro and lipid composition was compared. |
Institute | University of Hamburg |
Last Name | Martens |
First Name | Gerrit Alexander |
Address | Martin-Luther-King-Platz 3, 20146 Hamburg, Germany |
gerrit.alexander.martens@uni-hamburg.de | |
Phone | +49 40 42838-3934 |
Submit Date | 2022-11-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-12 |
Release Version | 1 |
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Collection:
Collection ID: | CO002441 |
Collection Summary: | Hooded seals (Cystophora cristata) were captured in the pack ice of the Greenland Sea under permits from relevant Norwegian and Greenland authorities. The adult hooded seals were euthanized immediately following capture, by sedation with intramuscular injection of zolazepam/tiletamine (1.5–2.0 mg per kg of body mass), followed by catheterization of the extradural intravertebral vein and i.v. injection of an overdose of pentobarbital (Euthasol vet., Le Vet B.V., Netherlands; 30 mg per kg of body mass). All animal handling was in accordance with the Norwegian Animal Welfare Act and with approvals from the National Animal Research Authority of Norway (permits no. 7247, 19305 and 22751). Fresh visual cortex samples from hooded seal adults were minced and placed in cooled (4 °C) artificial cerebrospinal fluid (aCSF; 128 mM NaCl, 3 mM KCl, 1.5 mM CaCl2, 1 mM MgCl2, 24 mM NaHCO3, 0.5 mM NaH2PO4, 20 mM sucrose, 10 mM D-glucose) saturated with 95% O2−5% CO2 (normoxia) and further processed in vitro. |
Sample Type: | Brain |
Storage Conditions: | -80℃ |