Summary of Study ST002511
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001621. The data can be accessed directly via it's Project DOI: 10.21228/M85M6X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002511 |
| Study Title | Enhanced niche colonization and competition during bacterial adaptation to a fungus |
| Study Type | Fungal / bacterial interaction |
| Study Summary | Enhanced niche 1 colonization and competition during bacterial adaptation to a fungus |
| Institute | Netherlands Institute of Ecology |
| Department | Microbial Ecology |
| Last Name | Tyc |
| First Name | Olaf |
| Address | Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands |
| Olaf.Tyc@kgu.de | |
| Phone | +496963018046 |
| Submit Date | 2023-03-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-04-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002604 |
| Collection Summary: | For analysis and trapping of volatile organic compounds two-compartment glass Petri-dishes (Garbeva et al., 2014) containing 1% LB-A (Carl-Roth LB- Agar) were applied. The fungus Aspergillus niger was inoculated 24 hours before addition of the bacterial strain on the left side of the two compartment glass petri-dish and incubated overnight at 28 °C. The bacterial strain Bacillus subtilis 3610 was incubated overnight at 28 °C in LB- liquid medium (Carl-Roth LB- Medium). After overnight _incubation a volume of 2 µl of the grown Bacillus subtilis 3610 culture was spotted on the opposite side of the Aspergillus niger culture. The glass Petri-dishes were kept open for 25 min. for drying of the droplets. After the drying process plates were closed by a lid with an outlet connected to a steel trap containing 150 mg Tenax TA and 150 mg Carbopack B (Markes International Ltd) and incubated at 28 °C. The Tenax steel traps were collected after three and five days of incubation and stored at 4 °C until GC-Q-TOF analysis. As controls, glass Petri-dishes containing LB agar media without inoculated bacteria or fungi were used. |
| Sample Type: | Fungal/bacterial volatiles |
| Collection Method: | Tenax Trap |
| Collection Location: | Wageningen, The Netherlands |
| Collection Frequency: | 3 days and 5 days |
| Collection Duration: | 5 min. |
| Storage Conditions: | 4℃ |
| Collection Vials: | Tenax |
| Storage Vials: | Metal tubes |
