Summary of Study ST000543

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000397. The data can be accessed directly via it's Project DOI: 10.21228/M8R013 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000543
Study TitleHigh Resolution orbittrap Mass Spectrometer to measure low abundance isotope enrichment in individual muscle proteins
Study Typeisotope encrichment and comparison of mass spectrometer platforms
Study SummaryStable isotope-labeled amino acids have long been used to measure the fractional synthesis rate of proteins, although the mass spectrometry platforms used for such analyses have changed throughout the years. More recently, tandem mass spectrometers such as triple quadrupoles have been accepted as the standard platform for enrichment measurement due to their sensitivity and the enhanced specificity offered by multiple reaction monitoring (MRM) experiments. The limit in the utility of such platforms for enrichment analysis occurs when measuring very low levels of enrichment from small amounts of sample, particularly proteins isolated from two-dimensional gel electrophoresis (2D-GE), where interference from contaminant ions impact the sensitivity of the measurement. We therefore applied a high resolution orbitrap mass spectrometer to the analysis of [ring-13C6]-phenylalanine enrichment in individual muscle proteins isolated with 2D-GE. Comparison of samples analyzed on both platforms revealed that the high resolution MS has significantly improved sensitivity relative to the triple quadrupole MS at very low-level enrichments due to its ability to resolve interferences in the m/z dimension.
Institute
Mayo Clinic
DepartmentEndocrinology
LaboratoryMayo Clinic Metabolomics Resource Core
Last NameNair
First NameSreekumaran
Address200 First Street SW, Rochester, MN 55905
EmailNair.K@mayo.edu
Phone507-285-2415
Submit Date2016-07-22
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Sreekumaran Nair Sreekumaran Nair
https://dx.doi.org/10.21228/M8R013
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id group time (mins)
SA028063R13A-180A 180
SA028064R5A-180A 180
SA028065R3A-180A 180
SA028066R17A-180A 180
SA028067R2A-180A 180
SA028068R15A-180A 180
SA028069R10A-180A 180
SA028070R12A-180A 180
SA028071R6A-180A 180
SA028072R16A-180A 180
SA028073R3A-480A 480
SA028074R17A-480A 480
SA028075R16A-480A 480
SA028076R13A-480A 480
SA028077R10A-480A 480
SA028078R6A-480A 480
SA028079R5A-480A 480
SA028080R15A-480A 480
SA028081R2A-480A 480
SA028082R12A-480A 480
SA028083R5B-180B 180
SA028084R2B-180B 180
SA028085R6B-180B 180
SA028086R3B-180B 180
SA028087R10B-180B 180
SA028088R16B-180B 180
SA028089R12B-180B 180
SA028090R13B-180B 180
SA028091R15B-180B 180
SA028092R17B-180B 180
SA028093R16B-480B 480
SA028094R5B-480B 480
SA028095R15B-480B 480
SA028096R10B-480B 480
SA028097R3B-480B 480
SA028098R2B-480B 480
SA028099R17B-480B 480
SA028100R13B-480B 480
SA028101R12B-480B 480
SA028102R6B-480B 480
SA028103R3C-180C 180
SA028104R2C-180C 180
SA028105R3C-480C 480
SA028106R2C-480C 480
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