Summary of Study ST000897
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000623. The data can be accessed directly via it's Project DOI: 10.21228/M84T25 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000897 |
Study Title | Untargeted metabolomics analysis of ischemia-reperfusion injured hearts ex vivo from sedentary and exercise trained rats. |
Study Summary | The effects of exercise on the heart and its resistance to disease are well-documented. Recent studies have identified exercise-induced resistance to arrhythmia is due to the preservation of mitochondrial membrane potential. To identify novel metabolic changes that occurred parallel to these mitochondrial alterations, we performed non-targeted metabolomics analysis on hearts from sedentary (Sed) and exercise- trained (Ex) rats challenged with isolated heart ischemia-reperfusion injury (I/R). Eight weeks old Sprague- Dawley rats were treadmill trained five days/week for six weeks (exercise duration and intensity progressively increased to 1 hour at 30 m/min up to 10.5% incline, 75-80% VO2mx). The recovery of pre-ischemic function for sedentary rat hearts was 28.8+/-5.4% (N=12) compared to exercise trained hearts which recovered 51.9%+/-5.7 (N=14)(p<0.001). Non-targeted GC-MS metabolomics analysis of 1) Sedentary rat hearts; 2) Exercise-trained rat hearts; 3) Sedentary rat hearts challenged with global ischemia-reperfusion (I/R) injury; and 4) Exercise-trained rat hearts challeged with global I/R (10/group) revealed 20 statistically significant metabolites between groups by ANOVA using Metaboanalyst (p<0.001). Enrichment analysis of these metabolites for pathway-associated metabolic sets indicated a >10 fold enrichment for ammonia recycling and protein biosynthesis (L-Glutamic acid; L-Proline; L-Histidine; L-Serine; L-Aspartic acid; L-Glutamine)(p<=4.05E-05, FDR=0.0024). Subsequent comparison of the sedentary hearts post-I/R and exercise-trained hearts post-I/R further identified significant differences in metabolites related to Aminoacyl-tRNA biosynthesis and nitrogen metabolism (4) (p<=1.24E-05, FDR<=5.07E-4). These studies shed light on novel mechanisms in which exercise-induced cardioprotection occurs in I/R which complement both the mitochondrial stabilization and antioxidant mechanisms recently described. These findings also link protein synthesis and protein degradation (protein quality control mechanisms) with exercise-linked cardioprotection and mitochondrial susceptibility for the first time in cardiac I/R. |
Institute | University of North Carolina at Chapel Hill |
Department | McAllister heart Institute, Department of Internal medicine |
Laboratory | Multiple Centers |
Last Name | Willis |
First Name | Monte |
Address | 111 Mason Farm road, Chapel Hill, North Carolina, 27599-7126, USA |
monte_willis@med.unc.edu | |
Phone | 919-360-7599 |
Submit Date | 2017-05-20 |
Study Comments | Cardiac tissue |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2018-02-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)
mb_sample_id | local_sample_id | Animal Group |
---|---|---|
SA052718 | H14 | Exercise Control |
SA052719 | H13 | Exercise Control |
SA052720 | H5 | Exercise Control |
SA052721 | H18 | Exercise Control |
SA052722 | H28 | Exercise Control |
SA052723 | H39 | Exercise Control |
SA052724 | H36 | Exercise Control |
SA052725 | H2 | Exercise Control |
SA052726 | H27 | Exercise Control |
SA052727 | H23 | Exercise Control |
SA052728 | H22 | Exercise Ischemia/Reperfusion Injury |
SA052729 | H29 | Exercise Ischemia/Reperfusion Injury |
SA052730 | H31 | Exercise Ischemia/Reperfusion Injury |
SA052731 | H38 | Exercise Ischemia/Reperfusion Injury |
SA052732 | H20 | Exercise Ischemia/Reperfusion Injury |
SA052733 | H9 | Exercise Ischemia/Reperfusion Injury |
SA052734 | H6 | Exercise Ischemia/Reperfusion Injury |
SA052735 | H11 | Exercise Ischemia/Reperfusion Injury |
SA052736 | H17 | Exercise Ischemia/Reperfusion Injury |
SA052737 | H40 | Exercise Ischemia/Reperfusion Injury |
SA052738 | H1 | Sedentary Control |
SA052739 | H25 | Sedentary Control |
SA052740 | H30 | Sedentary Control |
SA052741 | H32 | Sedentary Control |
SA052742 | H21 | Sedentary Control |
SA052743 | H19 | Sedentary Control |
SA052744 | H34 | Sedentary Control |
SA052745 | H8 | Sedentary Control |
SA052746 | H15 | Sedentary Control |
SA052747 | H4 | Sedentary Control |
SA052748 | H3 | Sedentary Ischemia/Reperfusion Injury |
SA052749 | H12 | Sedentary Ischemia/Reperfusion Injury |
SA052750 | H10 | Sedentary Ischemia/Reperfusion Injury |
SA052751 | H7 | Sedentary Ischemia/Reperfusion Injury |
SA052752 | H16 | Sedentary Ischemia/Reperfusion Injury |
SA052753 | H24 | Sedentary Ischemia/Reperfusion Injury |
SA052754 | H35 | Sedentary Ischemia/Reperfusion Injury |
SA052755 | H33 | Sedentary Ischemia/Reperfusion Injury |
SA052756 | H26 | Sedentary Ischemia/Reperfusion Injury |
SA052757 | H37 | Sedentary Ischemia/Reperfusion Injury |
Showing results 1 to 40 of 40 |