Summary of Study ST001158

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000776. The data can be accessed directly via it's Project DOI: 10.21228/M8CH5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001158
Study TitleThe gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion (part-II)
Study SummarySix week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute
Johns Hopkins University
DepartmentDepartment of Physiology
LaboratoryPluznick Lab
Last NamePluznick
First NameJennifer
Address725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Emailjpluznick@jhmi.edu
Phone410 614 4660
Submit Date2019-03-11
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Jennifer Pluznick Jennifer Pluznick
https://dx.doi.org/10.21228/M8CH5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample
SA080342JHOP-05731Feces | Treatment:Angiotensin II
SA080343JHOP-05730Feces | Treatment:Angiotensin II
SA080344JHOP-05732Feces | Treatment:Angiotensin II
SA080345JHOP-05740Feces | Treatment:Angiotensin II
SA080346JHOP-05739Feces | Treatment:Angiotensin II
SA080347JHOP-05729Feces | Treatment:Angiotensin II
SA080348JHOP-05728Feces | Treatment:Angiotensin II
SA080349JHOP-05744Feces | Treatment:Angiotensin II
SA080350JHOP-05743Feces | Treatment:Angiotensin II
SA080351JHOP-05742Feces | Treatment:Angiotensin II
SA080352JHOP-05727Feces | Treatment:Angiotensin II
SA080353JHOP-05741Feces | Treatment:Angiotensin II
SA080354JHOP-05738Feces | Treatment:Saline
SA080355JHOP-05737Feces | Treatment:Saline
SA080356JHOP-05733Feces | Treatment:Saline
SA080357JHOP-05724Feces | Treatment:Saline
SA080358JHOP-05723Feces | Treatment:Saline
SA080359JHOP-05722Feces | Treatment:Saline
SA080360JHOP-05725Feces | Treatment:Saline
SA080361JHOP-05726Feces | Treatment:Saline
SA080362JHOP-05735Feces | Treatment:Saline
SA080363JHOP-05734Feces | Treatment:Saline
SA080364JHOP-05721Feces | Treatment:Saline
SA080365JHOP-05736Feces | Treatment:Saline
Showing results 1 to 24 of 24
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