Summary of Study ST001393
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001393 |
Study Title | Sea-ice diatom compatible solute shifts |
Study Type | Compatible solutes were quantified in sea-ice diatoms |
Study Summary | Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
Institute | University of Washington |
Department | School of Oceanography |
Laboratory | Ingalls Lab |
Last Name | Dawson |
First Name | Hannah |
Address | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195 |
hmdawson@uw.edu | |
Phone | 2062216750 |
Submit Date | 2020-03-24 |
Publications | Dawson et al., Elementa |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-09-29 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Other; Subject species: Nitzschia lecointei (Factor headings shown in green)
mb_sample_id | local_sample_id | Type | Salinity | Temp_degC |
---|---|---|---|---|
SA113432 | MediaBlk_ppt32 | Blk | 32 | NA |
SA113433 | MediaBlk_ppt40 | Blk | 40 | NA |
SA113434 | ASWFilterBlk_1 | Blk | NA | NA |
SA113435 | ASWFilterBlk_2 | Blk | NA | NA |
SA113436 | ASWFilterBlk_3 | Blk | NA | NA |
SA113437 | 32ppt-1C_A | Smp | 32 | -1 |
SA113438 | 32ppt-1C_B | Smp | 32 | -1 |
SA113439 | 32ppt-1C_C | Smp | 32 | -1 |
SA113440 | 32ppt4C_C | Smp | 32 | 4 |
SA113441 | 32ppt4C_A | Smp | 32 | 4 |
SA113442 | 32ppt4C_B | Smp | 32 | 4 |
SA113443 | 40ppt-1C_B | Smp | 40 | -1 |
SA113444 | 40ppt-1C_A | Smp | 40 | -1 |
SA113445 | 40ppt-1C_C | Smp | 40 | -1 |
SA113446 | 40ppt4C_B | Smp | 40 | 4 |
SA113447 | 40ppt4C_C | Smp | 40 | 4 |
SA113448 | 40ppt4C_A | Smp | 40 | 4 |
SA113449 | S2C_6 | Smp | NA | NA |
SA113450 | S2C_5 | Smp | NA | NA |
SA113451 | S2C_4 | Smp | NA | NA |
Showing results 1 to 20 of 20 |