Summary of Study ST002152
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001365. The data can be accessed directly via it's Project DOI: 10.21228/M88Q5H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002152 |
Study Title | Metabolomics analysis of mouse liver with or without SIRT5 deficiency |
Study Summary | 8 Wild-type (WT) and 8 Sirt5-/- (SIRT5 KO) mice on 129 background were maintained on a standard chow diet (Teklad Global 18% Protein Rodent Diet, ENVIGO, Cat.#2018) until they were put on euthanized. Liver metabolites were extracted (5-10 mg) were extracted using 80% methanol/water as the extraction solvent. Metabolite extract was split into two tubes (one for polar metabolite analysis and the other one for acyl-CoA analysis), and then extraction solvent was evaporated using a speed vacuum concentrator. Dry pellets were stored in −80 °C freezer until ready for LC-MS analysis. For acyl-CoA analysis, dry pellets were reconstituted into 30 μL of sample solvent (water containing 50 mM ammonium acetate) per 6 mg tissue, and 8 μL was injected into the LC-MS. For non-acyl-CoA polar metabolite analysis, pellets were reconstituted into 30 μL of sample solvent (water:methanol:acetonitrile, 2:1:1, v/v/v) per 3 mg tissue, and 3 μL was injected into the LC-MS. |
Institute | North Carolina State University |
Last Name | Liu |
First Name | Xiaojing |
Address | Polk Hall, RM 128 |
xliu68@ncsu.edu | |
Phone | 9195154387 |
Submit Date | 2022-04-14 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-05-09 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample Source |
---|---|---|
SA206325 | KO_6.raw | SIRT5 knockout |
SA206326 | KO_5.raw | SIRT5 knockout |
SA206327 | KO_4.raw | SIRT5 knockout |
SA206328 | KO_7.raw | SIRT5 knockout |
SA206329 | KO_8.raw | SIRT5 knockout |
SA206330 | KO_1_luna.raw | SIRT5 knockout |
SA206331 | KO_2_luna.raw | SIRT5 knockout |
SA206332 | KO_3.raw | SIRT5 knockout |
SA206333 | KO_2.raw | SIRT5 knockout |
SA206334 | KO_7_luna.raw | SIRT5 knockout |
SA206335 | KO_8_luna.raw | SIRT5 knockout |
SA206336 | KO_6_luna.raw | SIRT5 knockout |
SA206337 | KO_5_luna.raw | SIRT5 knockout |
SA206338 | KO_1.raw | SIRT5 knockout |
SA206339 | KO_3_luna.raw | SIRT5 knockout |
SA206340 | KO_4_luna.raw | SIRT5 knockout |
SA206341 | WT_7_luna.raw | Wild type |
SA206342 | WT_8_luna.raw | Wild type |
SA206343 | WT_6_luna.raw | Wild type |
SA206344 | WT_1_luna.raw | Wild type |
SA206345 | WT_5.raw | Wild type |
SA206346 | WT_6.raw | Wild type |
SA206347 | WT_4.raw | Wild type |
SA206348 | WT_3.raw | Wild type |
SA206349 | WT_2.raw | Wild type |
SA206350 | WT_7.raw | Wild type |
SA206351 | WT_8.raw | Wild type |
SA206352 | WT_4_luna.raw | Wild type |
SA206353 | WT_3_luna.raw | Wild type |
SA206354 | WT_2_luna.raw | Wild type |
SA206355 | WT_1.raw | Wild type |
SA206356 | WT_5_luna.raw | Wild type |
Showing results 1 to 32 of 32 |