Summary of Study ST002366
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001521. The data can be accessed directly via it's Project DOI: 10.21228/M83707 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002366 |
| Study Title | Single cell lipidome analysis of phosphatidylcholines and spingomyelins from HepG2 and C2C12 cells |
| Study Type | Quantitative single cell lipidomics |
| Study Summary | We have developed a high-throughput method for the detection and quantification of a wide range of phosphatidylcholine (PC) and sphingomyelin (SM) species from single cells that combines fluorescence-assisted cell sorting (FACS) with automated chip-based nanoelectrospray ionization (nanoESI) and shotgun lipidomics. Using this method we can detect and perform relative quantitation on more than >50 different PC and SM species from immortalised human cells, and can easily distinguish between cells of different lineages (e.g. hepatocarcinoma HePG2 vs C2C12 myoblasts) and cells treated with exogenous fatty acids. |
| Institute | Victor Chang Cardiac Research Institute |
| Laboratory | Cellular Bioenergetics Laboratory |
| Last Name | Hancock |
| First Name | Sarah |
| Address | Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia |
| s.hancock@victorchang.edu.au | |
| Phone | +61414537526 |
| Submit Date | 2022-10-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2023-10-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA237061 | HepG2-H08 | CON | cell number:bulk_extract | type:sample |
| SA237062 | HepG2-H09 | CON | cell number:bulk_extract | type:sample |
| SA237063 | HepG2-H07 | CON | cell number:bulk_extract | type:sample |
| SA237064 | C2C12-H07 | CON | cell number:bulk_extract | type:sample |
| SA237065 | C2C12-H09 | CON | cell number:bulk_extract | type:sample |
| SA237066 | C2C12-H08 | CON | cell number:bulk_extract | type:sample |
| SA237067 | HepG2-A01 | CON | cell number:fifty_cells | type:sample |
| SA237068 | C2C12-A02 | CON | cell number:fifty_cells | type:sample |
| SA237069 | C2C12-A03 | CON | cell number:fifty_cells | type:sample |
| SA237070 | C2C12-A01 | CON | cell number:fifty_cells | type:sample |
| SA237071 | HepG2-A03 | CON | cell number:fifty_cells | type:sample |
| SA237072 | HepG2-A02 | CON | cell number:fifty_cells | type:sample |
| SA237073 | HepG2-F02 | CON | cell number:single_cells | type:sample |
| SA237074 | HepG2-F03 | CON | cell number:single_cells | type:sample |
| SA237075 | C2C12-D01 | CON | cell number:single_cells | type:sample |
| SA237076 | HepG2-F01 | CON | cell number:single_cells | type:sample |
| SA237077 | C2C12-D02 | CON | cell number:single_cells | type:sample |
| SA237078 | C2C12-E01 | CON | cell number:single_cells | type:sample |
| SA237079 | C2C12-D03 | CON | cell number:single_cells | type:sample |
| SA237080 | C2C12-C03 | CON | cell number:single_cells | type:sample |
| SA237081 | HepG2-E02 | CON | cell number:single_cells | type:sample |
| SA237082 | HepG2-G01 | CON | cell number:single_cells | type:sample |
| SA237083 | C2C12-B03 | CON | cell number:single_cells | type:sample |
| SA237084 | C2C12-B02 | CON | cell number:single_cells | type:sample |
| SA237085 | C2C12-B01 | CON | cell number:single_cells | type:sample |
| SA237086 | C2C12-C01 | CON | cell number:single_cells | type:sample |
| SA237087 | HepG2-G03 | CON | cell number:single_cells | type:sample |
| SA237088 | HepG2-E01 | CON | cell number:single_cells | type:sample |
| SA237089 | HepG2-G02 | CON | cell number:single_cells | type:sample |
| SA237090 | C2C12-C02 | CON | cell number:single_cells | type:sample |
| SA237091 | HepG2-E03 | CON | cell number:single_cells | type:sample |
| SA237092 | C2C12-E02 | CON | cell number:single_cells | type:sample |
| SA237093 | HepG2-C02 | CON | cell number:single_cells | type:sample |
| SA237094 | HepG2-C03 | CON | cell number:single_cells | type:sample |
| SA237095 | C2C12-G02 | CON | cell number:single_cells | type:sample |
| SA237096 | C2C12-G03 | CON | cell number:single_cells | type:sample |
| SA237097 | HepG2-B01 | CON | cell number:single_cells | type:sample |
| SA237098 | HepG2-B02 | CON | cell number:single_cells | type:sample |
| SA237099 | HepG2-B03 | CON | cell number:single_cells | type:sample |
| SA237100 | C2C12-G01 | CON | cell number:single_cells | type:sample |
| SA237101 | HepG2-C01 | CON | cell number:single_cells | type:sample |
| SA237102 | C2C12-E03 | CON | cell number:single_cells | type:sample |
| SA237103 | C2C12-F03 | CON | cell number:single_cells | type:sample |
| SA237104 | C2C12-F01 | CON | cell number:single_cells | type:sample |
| SA237105 | HepG2-D03 | CON | cell number:single_cells | type:sample |
| SA237106 | C2C12-F02 | CON | cell number:single_cells | type:sample |
| SA237107 | HepG2-D01 | CON | cell number:single_cells | type:sample |
| SA237108 | HepG2-D02 | CON | cell number:single_cells | type:sample |
| SA237109 | HepG2-H10 | DHA | cell number:bulk_extract | type:sample |
| SA237110 | HepG2-H12 | DHA | cell number:bulk_extract | type:sample |
| SA237111 | C2C12-H10 | DHA | cell number:bulk_extract | type:sample |
| SA237112 | HepG2-H11 | DHA | cell number:bulk_extract | type:sample |
| SA237113 | C2C12-H11 | DHA | cell number:bulk_extract | type:sample |
| SA237114 | C2C12-H12 | DHA | cell number:bulk_extract | type:sample |
| SA237115 | HepG2-A08 | DHA | cell number:fifty_cells | type:sample |
| SA237116 | HepG2-A07 | DHA | cell number:fifty_cells | type:sample |
| SA237117 | C2C12-A09 | DHA | cell number:fifty_cells | type:sample |
| SA237118 | C2C12-A08 | DHA | cell number:fifty_cells | type:sample |
| SA237119 | C2C12-A07 | DHA | cell number:fifty_cells | type:sample |
| SA237120 | HepG2-A09 | DHA | cell number:fifty_cells | type:sample |
| SA237121 | C2C12-E09 | DHA | cell number:single_cells | type:sample |
| SA237122 | HepG2-D07 | DHA | cell number:single_cells | type:sample |
| SA237123 | HepG2-C09 | DHA | cell number:single_cells | type:sample |
| SA237124 | C2C12-E08 | DHA | cell number:single_cells | type:sample |
| SA237125 | HepG2-D08 | DHA | cell number:single_cells | type:sample |
| SA237126 | C2C12-D09 | DHA | cell number:single_cells | type:sample |
| SA237127 | HepG2-E07 | DHA | cell number:single_cells | type:sample |
| SA237128 | HepG2-D09 | DHA | cell number:single_cells | type:sample |
| SA237129 | HepG2-C08 | DHA | cell number:single_cells | type:sample |
| SA237130 | C2C12-E07 | DHA | cell number:single_cells | type:sample |
| SA237131 | C2C12-F08 | DHA | cell number:single_cells | type:sample |
| SA237132 | C2C12-G08 | DHA | cell number:single_cells | type:sample |
| SA237133 | C2C12-G09 | DHA | cell number:single_cells | type:sample |
| SA237134 | HepG2-B07 | DHA | cell number:single_cells | type:sample |
| SA237135 | C2C12-G07 | DHA | cell number:single_cells | type:sample |
| SA237136 | HepG2-B08 | DHA | cell number:single_cells | type:sample |
| SA237137 | C2C12-F09 | DHA | cell number:single_cells | type:sample |
| SA237138 | HepG2-C07 | DHA | cell number:single_cells | type:sample |
| SA237139 | HepG2-B09 | DHA | cell number:single_cells | type:sample |
| SA237140 | C2C12-F07 | DHA | cell number:single_cells | type:sample |
| SA237141 | C2C12-D08 | DHA | cell number:single_cells | type:sample |
| SA237142 | C2C12-B09 | DHA | cell number:single_cells | type:sample |
| SA237143 | HepG2-G07 | DHA | cell number:single_cells | type:sample |
| SA237144 | C2C12-B08 | DHA | cell number:single_cells | type:sample |
| SA237145 | C2C12-D07 | DHA | cell number:single_cells | type:sample |
| SA237146 | HepG2-G09 | DHA | cell number:single_cells | type:sample |
| SA237147 | HepG2-G08 | DHA | cell number:single_cells | type:sample |
| SA237148 | HepG2-F09 | DHA | cell number:single_cells | type:sample |
| SA237149 | C2C12-B07 | DHA | cell number:single_cells | type:sample |
| SA237150 | HepG2-E08 | DHA | cell number:single_cells | type:sample |
| SA237151 | HepG2-F08 | DHA | cell number:single_cells | type:sample |
| SA237152 | HepG2-F07 | DHA | cell number:single_cells | type:sample |
| SA237153 | HepG2-E09 | DHA | cell number:single_cells | type:sample |
| SA237154 | C2C12-C08 | DHA | cell number:single_cells | type:sample |
| SA237155 | C2C12-C07 | DHA | cell number:single_cells | type:sample |
| SA237156 | C2C12-C09 | DHA | cell number:single_cells | type:sample |
| SA237157 | HepG2-H06 | N/A | cell number:bulk_extract | type:blank |
| SA237158 | C2C12-H06 | N/A | cell number:bulk_extract | type:blank |
| SA237159 | C2C12-H02 | N/A | cell number:single_cells | type:blank |
| SA237160 | C2C12-H03 | N/A | cell number:single_cells | type:blank |
