Summary of Study ST002543

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001638. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX4D This work is supported by NIH grant, U2C- DK119886.

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Study IDST002543
Study TitleGC/MS analysis of hypoxic volatile metabolic markers in the MDA-MB-231 breast cancer cell line
Study SummaryHypoxia in disease describes persistent low oxygen conditions, observed in a range of pathologies, including cancer. In the discovery of biomarkers in biological models, pathophysiological traits present a source of translatable metabolic products for the diagnosis of disease in humans. Part of the metabolome is represented by its volatile, gaseous fraction; the volatilome. Human volatile profiles, such as those found in breath, are able to diagnose disease, however accurate volatile biomarker discovery is required to target reliable biomarkers to develop new diagnostic tools. Using custom chambers to control oxygen levels and facilitate headspace sampling, the MDA-MB-231 breast cancer cell line was exposed to hypoxia (1% oxygen) for 24 hours. The maintenance of hypoxic conditions in the system was successfully validated over this time period. Targeted and untargeted gas chromatography mass spectrometry approaches revealed four significantly altered volatile organic compounds when compared to control cells. Three compounds were actively consumed by cells: methyl chloride, acetone and n-Hexane. Cells under hypoxia also produced significant amounts of styrene. This work presents a novel methodology for identification of volatile metabolisms under controlled gas conditions with novel observations of volatile metabolisms by breast cancer cells.
Institute
University of York
Last NameIssitt
First NameTheo
AddressBiology Dept. University of York, Personal
Emailti538@york.ac.uk
Phone07398244497
Submit Date2023-03-31
Num Groups4
PublicationsT. Issitt et al., Volatile compounds in human breath: critical review and meta-analysis Journal of Breath Research, Volume 16, Number 2 (2022) https://iopscience.iop.org/article/10.1088/1752-7163/ac5230#jbrac5230s2
Analysis Type DetailGC-MS
Release Date2023-04-21
Release Version1
Theo Issitt Theo Issitt
https://dx.doi.org/10.21228/M8ZX4D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Treatment
SA255602c3Cells Control
SA255603c4Cells Control
SA255604c6Cells Control
SA255605c2Cells Control
SA255606c5Cells Control
SA255607c1Cells Control
SA255608h2Cells Hypoxia
SA255609h1Cells Hypoxia
SA255610h3Cells Hypoxia
SA255611h4Cells Hypoxia
SA255612h5Cells Hypoxia
SA255613h6Cells Hypoxia
SA255614m3Media Control
SA255615m2Media Control
SA255616m4Media Control
SA255617m5Media Control
SA255618m6Media Control
SA255619m1Media Control
SA255620hm4Media Hypoxia
SA255621hm3Media Hypoxia
SA255622hm5Media Hypoxia
SA255623hm6Media Hypoxia
SA255624hm2Media Hypoxia
SA255625hm1Media Hypoxia
Showing results 1 to 24 of 24
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