Summary of Study ST002905

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001773. The data can be accessed directly via it's Project DOI: 10.21228/M8J420 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002905
Study TitlePorphyrin analysis of K562 cells following C13-Serine and C13-Glycine tracing during folate depletion.
Study SummaryCulture of K562 cells for 7 days in RPMI media containing 2000 nM folic acid or 100 nM folic acid. At day 8, media was changed to 2000 nM or 100 nM folic acid with unlabeled serine and glycine, or 2-C13-Serine and 2-C13-Glycine at RPMI levels. Amino acid tracing was performed for 24 hours. These samples were followed up by metabolomics targeting porphyrin metabolites.
Institute
Boston Children's Hospital, Harvard Medical School
Departmentpathology
LaboratoryKanarek Lab
Last NameKanarek
First NameNaama
AddressEnders 1116.2, 300 Longwood Ave, Boston, MA 02115
Emailnaama.kanarek@childrens.harvard.edu
Phone6173557433
Submit Date2023-10-02
Num Groups5
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-02-13
Release Version1
Naama Kanarek Naama Kanarek
https://dx.doi.org/10.21228/M8J420
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA315921K562_100nM_FA_C13_Glycine_porphyrin_4100nM_FA_C13_Glycine
SA315922K562_100nM_FA_C13_Glycine_porphyrin_1100nM_FA_C13_Glycine
SA315923K562_100nM_FA_C13_Glycine_porphyrin_2100nM_FA_C13_Glycine
SA315924K562_100nM_FA_C13_Glycine_porphyrin_3100nM_FA_C13_Glycine
SA315925K562_100nM_FA_C13_Serine_porphyrin_1100nM_FA_C13_Serine
SA315926K562_100nM_FA_C13_Serine_porphyrin_2100nM_FA_C13_Serine
SA315927K562_100nM_FA_C13_Serine_porphyrin_4100nM_FA_C13_Serine
SA315928K562_100nM_FA_C13_Serine_porphyrin_3100nM_FA_C13_Serine
SA315929K562_100nM_FA_Unlabeled_porphyrin_3100nM_FA_Unlabeled
SA315930K562_100nM_FA_Unlabeled_porphyrin_1100nM_FA_Unlabeled
SA315931K562_100nM_FA_Unlabeled_porphyrin_2100nM_FA_Unlabeled
SA315932K562_100nM_FA_Unlabeled_porphyrin_4100nM_FA_Unlabeled
SA315933K562_2000nM_FA_C13_Glycine_porphyrin_12000nM_FA_C13_Glycine
SA315934K562_2000nM_FA_C13_Glycine_porphyrin_22000nM_FA_C13_Glycine
SA315935K562_2000nM_FA_C13_Glycine_porphyrin_32000nM_FA_C13_Glycine
SA315936K562_2000nM_FA_C13_Glycine_porphyrin_42000nM_FA_C13_Glycine
SA315937K562_2000nM_FA_C13_Serine_porphyrin_12000nM_FA_C13_Serine
SA315938K562_2000nM_FA_C13_Serine_porphyrin_42000nM_FA_C13_Serine
SA315939K562_2000nM_FA_C13_Serine_porphyrin_22000nM_FA_C13_Serine
SA315940K562_2000nM_FA_C13_Serine_porphyrin_32000nM_FA_C13_Serine
SA315941K562_2000nM_FA_Unlabeled_porphyrin_22000nM_FA_Unlabeled
SA315942K562_2000nM_FA_Unlabeled_porphyrin_32000nM_FA_Unlabeled
SA315943K562_2000nM_FA_Unlabeled_porphyrin_12000nM_FA_Unlabeled
SA315944K562_2000nM_FA_Unlabeled_porphyrin_42000nM_FA_Unlabeled
Showing results 1 to 24 of 24
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