Summary of Study ST002950

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001835. The data can be accessed directly via it's Project DOI: 10.21228/M8HT5Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002950
Study TitleInvestigate the impact of feeding time on the hexosamine biosynthetic pathway (HBP) in the mouse liver and heart using targeted metabolomics: biogenic amines
Study SummaryThe overall goal of this project is to advance our understanding of post-translational mechanisms that mediate metabolic regulation of time-of-day-specific protein functions to orchestrate daily rhythms and maintain homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) day-night cycles are key hallmarks of animal health span and are strongly regulated by circadian clocks. Circadian clocks are cell autonomous molecular timers present in the brain and in peripheral organs that enable animals to adapt to predictable daily changes in environment and regulate rhythmic processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, hormonal signaling and neuronal excitability. Besides light, the dominant time cue for the brain clock, metabolic signals from clock-controlled feeding-fasting cycles represent the most potent time cue to entrain and synchronize peripheral clocks in key organs. Much effort has been dedicated to understanding the metabolic regulation of daily biological rhythms, but many important mechanisms are only just emerging. We recently established that metabolic signals from feeding-fasting cycles regulate daily biological rhythms in Drosophila through rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which integrates the metabolites from glucose, amino acid, lipid and nucleotide metabolism. We now propose to investigate whether feeding activity can regulate daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of HBP metabolites in mouse liver and heart are affected by different feeding time within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and heart tissues every 4 hours over a 24-hour period. The liver and heart samples were subjected to targeted metabolomic analysis for HBP metabolites.
Institute
University of California, Davis
DepartmentDepartment of Entomology and Nematology
LaboratoryChiu lab
Last NameChiu
First NameJoanna
Address6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA
Emailjcchiu@ucdavis.edu
Phone(530) 752-1643
Submit Date2023-10-27
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-05-17
Release Version1
Joanna Chiu Joanna Chiu
https://dx.doi.org/10.21228/M8HT5Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Organ Treatment
SA321334Heart-ZT19-rep3-CHeart Control
SA321335Heart-ZT19-rep1-CHeart Control
SA321336Heart-ZT15-rep3-CHeart Control
SA321337Heart-ZT23-rep1-CHeart Control
SA321338Heart-ZT23-rep2-CHeart Control
SA321339Heart-ZT3-rep1-CHeart Control
SA321340Heart-ZT23-rep3-CHeart Control
SA321341Heart-ZT15-rep2-CHeart Control
SA321342Heart-ZT19-rep2-CHeart Control
SA321343Heart-ZT7-rep2-CHeart Control
SA321344Heart-ZT7-rep1-CHeart Control
SA321345Heart-ZT15-rep1-CHeart Control
SA321346Heart-ZT3-rep2-CHeart Control
SA321347Heart-ZT7-rep3-CHeart Control
SA321348Heart-ZT3-rep3-CHeart Control
SA321349Heart-ZT11-rep1-CHeart Control
SA321350Heart-ZT11-rep2-CHeart Control
SA321351Heart-ZT11-rep3-CHeart Control
SA321352Heart-ZT19-rep2-E-EHeart Expt
SA321353Heart-ZT15-rep3-E-EHeart Expt
SA321354Heart-ZT19-rep1-E-EHeart Expt
SA321355Heart-ZT19-rep3-E-EHeart Expt
SA321356Heart-ZT23-rep2-E-EHeart Expt
SA321357Heart-ZT15-rep2-E-EHeart Expt
SA321358Heart-ZT23-rep3-E-EHeart Expt
SA321359Heart-ZT23-rep1-E-EHeart Expt
SA321360Heart-ZT7-rep3-E-EHeart Expt
SA321361Heart-ZT3-rep3-E-EHeart Expt
SA321362Heart-ZT3-rep2-E-EHeart Expt
SA321363Heart-ZT15-rep1-E-EHeart Expt
SA321364Heart-ZT7-rep1-E-EHeart Expt
SA321365Heart-ZT7-rep2-E-EHeart Expt
SA321366Heart-ZT11-rep3-E-EHeart Expt
SA321367Heart-ZT11-rep2-E-EHeart Expt
SA321368Heart-ZT11-rep1-E-EHeart Expt
SA321369Heart-ZT3-rep1-EHeart Expt
SA321370PoolQC001-HHeart QC1
SA321371PoolQC002-HHeart QC1
SA321372PoolQC004-HHeart QC1
SA321373PoolQC003-HHeart QC1
SA321374Liver-ZT19-rep3-C-CLiver Control
SA321375Liver-ZT19-rep2-C-CLiver Control
SA321376Liver-ZT19-rep1-C-CLiver Control
SA321377Liver-ZT23-rep1-C-CLiver Control
SA321378Liver-ZT23-rep3-C-CLiver Control
SA321379Liver-ZT23-rep2-C-CLiver Control
SA321380Liver-ZT11-rep3-C-CLiver Control
SA321381Liver-ZT3-rep2-C-CLiver Control
SA321382Liver-ZT7-rep1-C-CLiver Control
SA321383Liver-ZT7-rep2-C-CLiver Control
SA321384Liver-ZT15-rep3-C-CLiver Control
SA321385Liver-ZT3-rep1-C-CLiver Control
SA321386Liver-ZT3-rep3-C-CLiver Control
SA321387Liver-ZT15-rep1-C-CLiver Control
SA321388Liver-ZT7-rep3-C-CLiver Control
SA321389Liver-ZT15-rep2-C-CLiver Control
SA321390Liver-ZT11-rep2-C-CLiver Control
SA321391Liver-ZT11-rep1-C-CLiver Control
SA321392Liver-ZT19-rep2-E-ELiver Expt
SA321393Liver-ZT19-rep1-E-ELiver Expt
SA321394Liver-ZT23-rep3-E-ELiver Expt
SA321395Liver-ZT15-rep3-E-ELiver Expt
SA321396Liver-ZT23-rep2-E-ELiver Expt
SA321397Liver-ZT23-rep1-E-ELiver Expt
SA321398Liver-ZT19-rep3-E-ELiver Expt
SA321399Liver-ZT3-rep3-E-ELiver Expt
SA321400Liver-ZT7-rep1-E-ELiver Expt
SA321401Liver-ZT3-rep2-E-ELiver Expt
SA321402Liver-ZT3-rep1-E-ELiver Expt
SA321403Liver-ZT15-rep2-E-ELiver Expt
SA321404Liver-ZT7-rep2-E-ELiver Expt
SA321405Liver-ZT7-rep3-E-ELiver Expt
SA321406Liver-ZT15-rep1-E-ELiver Expt
SA321407Liver-ZT11-rep3-E-ELiver Expt
SA321408Liver-ZT11-rep2-E-ELiver Expt
SA321409Liver-ZT11-rep1-E-ELiver Expt
SA321410PoolQC004-LLiver QC2
SA321411PoolQC002-LLiver QC2
SA321412PoolQC003-LLiver QC2
SA321413PoolQC001-LLiver QC2
SA321414MtdBlank003-HQC3 QC3
SA321415MtdBlank004-HQC3 QC3
SA321416MtdBlank002-HQC3 QC3
SA321417MtdBlank001-HQC3 QC3
SA321418MtdBlank004-LQC4 QC4
SA321419MtdBlank002-LQC4 QC4
SA321420MtdBlank001-LQC4 QC4
SA321421MtdBlank003-LQC4 QC4
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