Summary of Study ST003250

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002011. The data can be accessed directly via it's Project DOI: 10.21228/M8P23X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003250
Study TitleLipidomic analysis of Axon Regeneration in Xenopus laevis Tectum
Study SummaryCNS injuries of the anuran amphibian, Xenopus laevis, are uniquely befitted for studying the molecular compositions of neuronal regeneration of retinal ganglion cells (RGC) due to a functional recovery of optic axons disparate to adult mammalian analogues. RGCs and their optic nerve axons undergo irreversible neurodegeneration in glaucoma and associated optic neuropathies, resulting in blindness in mammals. Conversely, Xenopus demonstrates RGC lifetime-spanning regenerative capabilities after optic nerve crush, inciting opportunities to compare de novo regeneration and develop efficient pharmaceutical approaches for vision restoration. Studies revealing lipidome alterations during optic nerve regeneration are sparse and could serve as a solid foundation for these underlying molecular changes. We profile the lipid changes in a transgenic line of 1 year old Xenopus laevis Tg(islet2b:gfp) frogs that were either had a monocular surgery of either a left optic crush injury (crush) or sham surgery (sham). The matching controls of uninjured right optic nerves were also collected (control). Tg(islet2b:gfp) frogs were allowed to recover for 12 and 27 days post optic nerve crush. Following euthanasia, the optic nerves were collected for lipidomic analysis. A modified Bligh and Dyer method was used for lipid extraction, followed by untargeted mass spectrometry lipid profiling with a Q-Exactive Orbitrap Liquid Chromatography-Mass Spectrometer (LC MS-MS) coupled with Vanquish Horizon Binary UHPLC LC-MS system.
Institute
University of Miami
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Room 706-A, Miami, FL 33136
Emailsbhattacharya@med.miami.edu
Phone3054824103
Submit Date2024-01-16
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-06-24
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M8P23X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Amphibian; Subject species: Xenopus laevis (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment
SA353500CTL_27dpi_T_2Tectum Control
SA353501CTL_12dpi_T1Tectum Control
SA353502CTL_12dpi_T2Tectum Control
SA353503CTL_27dpi_T_3Tectum Control
SA353504CX_27dpi_T_2Tectum Crush
SA353505CX_12dpi_T_1Tectum Crush
SA353506CX_12dpi_T2Tectum Crush
SA353507CX_27dpi_T1Tectum Crush
SA353508CX_27dpi_T3Tectum Crush
SA353509L_T_Sham_2Tectum Sham
SA353510Left_T_Sham_1Tectum Sham
SA353511Right_T_Sham_2Tectum Sham
SA353512Right_T_Sham_1Tectum Sham
Showing results 1 to 13 of 13
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