Summary of Study ST003261

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002023. The data can be accessed directly via it's Project DOI: 10.21228/M8423Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003261
Study TitleExploration of RSL3 or Chlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complex-induced changes in the phospholipid oxidation of MDA-MB-231 breast cancer cells
Study SummaryChlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complexes (SCs) exhibit potent anti-cancer properties through incompletely understood molecular mechanisms. Here, we treated human MDA-MB-231 triple-negative breast cancer cells with the glutathione peroxidase (GPX)4 inhibitor RSL3 or chlorido[N,N’-disalicylidene-1,2-phenylenediamine]iron(III) complexes (SCs) and analyzed their oxidized phospholipid profile by targeted lipidomics. SCs induce extensive (hydroper)oxidation of arachidonic acid and adrenic acid in membrane phospholipids, particularly phosphatidylethanolamines (PE) and phosphatidylinositols (PC). In this process, SCs have demonstrated superior efficacy compared to the GPX4 inhibitor RSL3, an established ferroptosis inducer. Please note that one sample set was measured three times with the same sample-ID, but with different methods (oxPE, oxPC, oxPI), therefore each sub-class has their own raw-data file marked by their corresponding abbreviation (oxPE, oxPC, oxPI; e.g. "210309_MDA_Timecourse_RSL3_oxPE_dil_UD_Std_1ul_SFT.wiff", "210324_Rescue_Gust_compounds_oxPE_dil_UD_std_1ul_SFT.wiff", "210309_MDA_Timecourse_RSL3_oxPC_dil_UD_Std_1ul_SFT.wiff", "210324_Rescue_Gust_compounds_oxPC_dil_UD_std_1ul_SFT.wiff" or "210324_Rescue_Gust_compounds_oxPI_dil_UD_std_1ul_SFT.wiff", ).
Institute
University of Innsbruck
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
EmailAndreas.Koeberle@uibk.ac.at
Phone+43 512 507 57903
Submit Date2024-06-12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-06-27
Release Version1
Andreas Koeberle Andreas Koeberle
https://dx.doi.org/10.21228/M8423Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Treatment time point
SA354175210324_Rescue_Gust_compounds_2h_n2_Ti41_10uM_T58Breast cancer cells 10 µM Comp. 1 2 h
SA354176210324_Rescue_Gust_compounds_2h_n1_Ti41_10uM_T46Breast cancer cells 10 µM Comp. 1 2 h
SA354177210324_Rescue_Gust_compounds_2h_n3_Ti41_10uM_T70Breast cancer cells 10 µM Comp. 1 2 h
SA354178210324_Rescue_Gust_compounds_2h_n1_Ti41_F_10uM_T49Breast cancer cells 10 µM Comp. 2 2 h
SA354179210324_Rescue_Gust_compounds_2h_n2_Ti41_F_10uM_T61Breast cancer cells 10 µM Comp. 2 2 h
SA354180210324_Rescue_Gust_compounds_2h_n3_Ti41_F_10uM_T73Breast cancer cells 10 µM Comp. 2 2 h
SA354181210324_Rescue_Gust_compounds_2h_n3_Ti41_Cl_10uM_T76Breast cancer cells 10 µM Comp. 3 2 h
SA354182210324_Rescue_Gust_compounds_2h_n2_Ti41_Cl_10uM_T64Breast cancer cells 10 µM Comp. 3 2 h
SA354183210324_Rescue_Gust_compounds_2h_n1_Ti41_Cl_10uM_T52Breast cancer cells 10 µM Comp. 3 2 h
SA354187210309_MDA_Timecourse_RSL3_n2_24h_RSL3_10uM_24Breast cancer cells 10 µM RSL3 24 h
SA354188210309_MDA_Timecourse_RSL3_n3_24h_RSL3_10uM_36Breast cancer cells 10 µM RSL3 24 h
SA354189210309_MDA_Timecourse_RSL3_n1_24h_RSL3_10uM_12Breast cancer cells 10 µM RSL3 24 h
SA354184210309_MDA_Timecourse_RSL3_n3_2h_RSL3_10uM_27Breast cancer cells 10 µM RSL3 2 h
SA354185210309_MDA_Timecourse_RSL3_n2_2h_RSL3_10uM_15Breast cancer cells 10 µM RSL3 2 h
SA354186210309_MDA_Timecourse_RSL3_n1_2h_RSL3_10uM_3Breast cancer cells 10 µM RSL3 2 h
SA354190210309_MDA_Timecourse_RSL3_n3_4h_RSL3_10uM_30Breast cancer cells 10 µM RSL3 4 h
SA354191210309_MDA_Timecourse_RSL3_n2_4h_RSL3_10uM_18Breast cancer cells 10 µM RSL3 4 h
SA354192210309_MDA_Timecourse_RSL3_n1_4h_RSL3_10uM_6Breast cancer cells 10 µM RSL3 4 h
SA354193210309_MDA_Timecourse_RSL3_n2_6h_RSL3_10uM_21Breast cancer cells 10 µM RSL3 6 h
SA354194210309_MDA_Timecourse_RSL3_n3_6h_RSL3_10uM_33Breast cancer cells 10 µM RSL3 6 h
SA354195210309_MDA_Timecourse_RSL3_n1_6h_RSL3_10uM_9Breast cancer cells 10 µM RSL3 6 h
SA354148210324_Rescue_Gust_compounds_2h_n1_Ti41_1uM_T44Breast cancer cells 1 µM Comp. 1 2 h
SA354149210324_Rescue_Gust_compounds_2h_n3_Ti41_1uM_T68Breast cancer cells 1 µM Comp. 1 2 h
SA354150210324_Rescue_Gust_compounds_2h_n2_Ti41_1uM_T56Breast cancer cells 1 µM Comp. 1 2 h
SA354151210324_Rescue_Gust_compounds_2h_n2_Ti41_F_1uM_T59Breast cancer cells 1 µM Comp. 2 2 h
SA354152210324_Rescue_Gust_compounds_2h_n3_Ti41_F_1uM_T71Breast cancer cells 1 µM Comp. 2 2 h
SA354153210324_Rescue_Gust_compounds_2h_n1_Ti41_F_1uM_T47Breast cancer cells 1 µM Comp. 2 2 h
SA354154210324_Rescue_Gust_compounds_2h_n2_Ti41_Cl_1uM_T62Breast cancer cells 1 µM Comp. 3 2 h
SA354155210324_Rescue_Gust_compounds_2h_n3_Ti41_Cl_1uM_T74Breast cancer cells 1 µM Comp. 3 2 h
SA354156210324_Rescue_Gust_compounds_2h_n1_Ti41_Cl_1uM_T50Breast cancer cells 1 µM Comp. 3 2 h
SA354157210324_Rescue_Gust_compounds_2h_n3_RSL3_1uM_Ferrostatin1_3uM_T67Breast cancer cells 1 µM RSL3 + 3 µM Ferrostatin 2 h
SA354158210324_Rescue_Gust_compounds_2h_n1_RSL3_1uM_Ferrostatin1_3uM_T43Breast cancer cells 1 µM RSL3 + 3 µM Ferrostatin 2 h
SA354159210324_Rescue_Gust_compounds_2h_n2_RSL3_1uM_Ferrostatin1_3uM_T55Breast cancer cells 1 µM RSL3 + 3 µM Ferrostatin 2 h
SA354166210309_MDA_Timecourse_RSL3_n3_24h_RSL3_1uM_35Breast cancer cells 1 µM RSL3 24 h
SA354167210309_MDA_Timecourse_RSL3_n2_24h_RSL3_1uM_23Breast cancer cells 1 µM RSL3 24 h
SA354168210309_MDA_Timecourse_RSL3_n1_24h_RSL3_1uM_11Breast cancer cells 1 µM RSL3 24 h
SA354160210324_Rescue_Gust_compounds_2h_n2_RSL3_1uM_T54Breast cancer cells 1 µM RSL3 2 h
SA354161210324_Rescue_Gust_compounds_2h_n3_RSL3_1uM_T66Breast cancer cells 1 µM RSL3 2 h
SA354162210309_MDA_Timecourse_RSL3_n3_2h_RSL3_1uM_26Breast cancer cells 1 µM RSL3 2 h
SA354163210309_MDA_Timecourse_RSL3_n1_2h_RSL3_1uM_2Breast cancer cells 1 µM RSL3 2 h
SA354164210324_Rescue_Gust_compounds_2h_n1_RSL3_1uM_T42Breast cancer cells 1 µM RSL3 2 h
SA354165210309_MDA_Timecourse_RSL3_n2_2h_RSL3_1uM_14Breast cancer cells 1 µM RSL3 2 h
SA354169210309_MDA_Timecourse_RSL3_n3_4h_RSL3_1uM_29Breast cancer cells 1 µM RSL3 4 h
SA354170210309_MDA_Timecourse_RSL3_n1_4h_RSL3_1uM_5Breast cancer cells 1 µM RSL3 4 h
SA354171210309_MDA_Timecourse_RSL3_n2_4h_RSL3_1uM_17Breast cancer cells 1 µM RSL3 4 h
SA354172210309_MDA_Timecourse_RSL3_n3_6h_RSL3_1uM_32Breast cancer cells 1 µM RSL3 6 h
SA354173210309_MDA_Timecourse_RSL3_n1_6h_RSL3_1uM_8Breast cancer cells 1 µM RSL3 6 h
SA354174210309_MDA_Timecourse_RSL3_n2_6h_RSL3_1uM_20Breast cancer cells 1 µM RSL3 6 h
SA354196210324_Rescue_Gust_compounds_2h_n1_Ti41_3uM_T45Breast cancer cells 3 µM Comp. 1 2 h
SA354197210324_Rescue_Gust_compounds_2h_n3_Ti41_3uM_T69Breast cancer cells 3 µM Comp. 1 2 h
SA354198210324_Rescue_Gust_compounds_2h_n2_Ti41_3uM_T57Breast cancer cells 3 µM Comp. 1 2 h
SA354199210324_Rescue_Gust_compounds_2h_n1_Ti41_F_3uM_T48Breast cancer cells 3 µM Comp. 2 2 h
SA354200210324_Rescue_Gust_compounds_2h_n3_Ti41_F_3uM_T72Breast cancer cells 3 µM Comp. 2 2 h
SA354201210324_Rescue_Gust_compounds_2h_n2_Ti41_F_3uM_T60Breast cancer cells 3 µM Comp. 2 2 h
SA354202210324_Rescue_Gust_compounds_2h_n1_Ti41_Cl_3uM_T51Breast cancer cells 3 µM Comp. 3 2 h
SA354203210324_Rescue_Gust_compounds_2h_n3_Ti41_Cl_3uM_T75Breast cancer cells 3 µM Comp. 3 2 h
SA354204210324_Rescue_Gust_compounds_2h_n2_Ti41_Cl_3uM_T63Breast cancer cells 3 µM Comp. 3 2 h
SA354211210309_MDA_Timecourse_RSL3_n3_24h_DMSO_34Breast cancer cells DMSO 24 h
SA354212210309_MDA_Timecourse_RSL3_n2_24h_DMSO_22Breast cancer cells DMSO 24 h
SA354213210309_MDA_Timecourse_RSL3_n1_24h_DMSO_10Breast cancer cells DMSO 24 h
SA354205210324_Rescue_Gust_compounds_2h_n3_DMSO_T65Breast cancer cells DMSO 2 h
SA354206210309_MDA_Timecourse_RSL3_n1_2h_DMSO_1Breast cancer cells DMSO 2 h
SA354207210324_Rescue_Gust_compounds_2h_n2_DMSO_T53Breast cancer cells DMSO 2 h
SA354208210309_MDA_Timecourse_RSL3_n3_2h_DMSO_25Breast cancer cells DMSO 2 h
SA354209210309_MDA_Timecourse_RSL3_n2_2h_DMSO_13Breast cancer cells DMSO 2 h
SA354210210324_Rescue_Gust_compounds_2h_n1_DMSO_T41Breast cancer cells DMSO 2 h
SA354214210309_MDA_Timecourse_RSL3_n3_4h_DMSO_28Breast cancer cells DMSO 4 h
SA354215210309_MDA_Timecourse_RSL3_n2_4h_DMSO_16Breast cancer cells DMSO 4 h
SA354216210309_MDA_Timecourse_RSL3_n1_4h_DMSO_4Breast cancer cells DMSO 4 h
SA354217210309_MDA_Timecourse_RSL3_n3_6h_DMSO_31Breast cancer cells DMSO 6 h
SA354218210309_MDA_Timecourse_RSL3_n2_6h_DMSO_19Breast cancer cells DMSO 6 h
SA354219210309_MDA_Timecourse_RSL3_n1_6h_DMSO_7Breast cancer cells DMSO 6 h
Showing results 1 to 72 of 72
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