Summary of Study ST003306

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002056. The data can be accessed directly via it's Project DOI: 10.21228/M8VG0G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003306
Study TitleASCT2 is a major contributor to serine uptake in cancer cells
Study SummaryThe non-essential amino acid serine is a critical nutrient for cancer cells due to its diverse biosynthetic functions. While some tumors can synthesize serine de novo, others are auxotrophic and therefore reliant on serine uptake. Importantly, despite several transporters being known to be capable of transporting serine, the transporter(s) that mediate serine uptake in cancer cells are not known. Here, we characterize the amino acid transporter ASCT2 (SLC1A5) as a major contributor to serine uptake in cancer cells. ASCT2 is well-known as a glutamine transporter in cancer, and our work demonstrates that serine and glutamine compete for uptake through ASCT2. We further show that ASCT2-mediated serine uptake is essential for purine nucleotide biosynthesis and that ERĪ± promotes serine uptake by directly activating SLC1A5 transcription. Together, our work defines an additional important role for ASCT2 as a serine transporter in cancer and evaluates ASCT2 as a potential therapeutic target.
Institute
University of Illinois Chicago
DepartmentPhysiology and Biophysics
LaboratoryColoff Lab
Last NameConger
First NameKelly
Address909 S Wolcott Ave, Chicago, IL, 60612
Emailkconge2@uic.edu
Phone2314320406
Submit Date2024-06-17
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2024-07-15
Release Version1
Kelly Conger Kelly Conger
https://dx.doi.org/10.21228/M8VG0G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype Treatment
SA358636SLC1A5-3,2Human breast cancer cells ASCT2-KO 285uM Serine
SA358637SLC1A5-3,3Human breast cancer cells ASCT2-KO 285uM Serine
SA358638SLC1A5-1,1Human breast cancer cells ASCT2-KO 285uM Serine
SA358639SLC1A5-1,2Human breast cancer cells ASCT2-KO 285uM Serine
SA358640SLC1A5-1,3Human breast cancer cells ASCT2-KO 285uM Serine
SA358641SLC1A5-3,1Human breast cancer cells ASCT2-KO 285uM Serine
SA358642sgSLC1A5-1 IM 1Human breast cancer cells ASCT2-KO 50uM Serine
SA358643sgSLC1A5-1 IM 2Human breast cancer cells ASCT2-KO 50uM Serine
SA358644sgSLC1A5-1 IM 3Human breast cancer cells ASCT2-KO 50uM Serine
SA358645sgSLC1A5-3 IM 1Human breast cancer cells ASCT2-KO 50uM Serine
SA358646sgSLC1A5-3 IM 2Human breast cancer cells ASCT2-KO 50uM Serine
SA358647sgSLC1A5-3 IM 3Human breast cancer cells ASCT2-KO 50uM Serine
SA358651Luc Puro 3Human breast cancer cells Ctrl 285uM Serine
SA358652Luc Puro 1Human breast cancer cells Ctrl 285uM Serine
SA358653Luc Puro 2Human breast cancer cells Ctrl 285uM Serine
SA358654sgLuc IM 2Human breast cancer cells Ctrl 50uM Serine
SA358655sgLuc IM 3Human breast cancer cells Ctrl 50uM Serine
SA358656sgLuc IM 1Human breast cancer cells Ctrl 50uM Serine
SA358648sgLuc -Gln 60 3Human breast cancer cells Ctrl (-Gln)1hr
SA358649sgLuc -Gln 60 2Human breast cancer cells Ctrl (-Gln)1hr
SA358650sgLuc -Gln 60 1Human breast cancer cells Ctrl (-Gln)1hr
Showing results 1 to 21 of 21
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