Summary of Study ST001476

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001000. The data can be accessed directly via it's Project DOI: 10.21228/M8F982 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study ID	ST001476
Study Title	Design of Experiments for Maximizing T cell endpoints
Study Summary	Purified T cells from a single healthy donor were expanded in vitro with either magnetic beads or degradable micro-scaffold (DMS) particles. Magnetic bead cultures were expanded according to manufacturer’s instructions, while DMS cultures were expanded with varying DMS particle concentration, IL2 concentration, and antigen surface density on the particles, according to a design of experiments. Media of each culture of was sampled repeatedly over the course of a 14 day period. Quantities and characteristics of activated T cells were assessed at the end of the culture period, and media was analyzed by 1H-NMR. Analysis of spectra resulted in a set of 20 features that was used in predictive modeling of T cell endpoints, along with culture parameters described above and cytokine data. A second validation experiment was performed with different values for culture parameters, using the same culture, sampling, and NMR analysis methods.
Institute	University of Georgia
Department	Complex Carbohydrate Research Center
Laboratory	Edison
Last Name	Colonna
First Name	Max
Address	315 Riverbend Rd
Email	maxwellbaca@uga.edu
Phone	7065420257
Submit Date	2020-08-12
Num Groups	2
Total Subjects	1
Raw Data Available	Yes
Analysis Type Detail	NMR
Release Date	2020-09-10
Release Version	1

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	method
SA124598	batch1_92	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124599	batch1_67	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124600	batch1_103	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124601	batch1_32	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124602	batch1_72	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124603	batch1_118	Beads \| timepoint:11 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124604	batch1_20	Beads \| timepoint:14 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124605	batch1_78	Beads \| timepoint:14 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124606	batch1_11	Beads \| timepoint:14 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124607	batch1_2	Beads \| timepoint:14 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124608	batch1_36	Beads \| timepoint:14 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124609	batch1_26	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124610	batch1_39	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124611	batch1_119	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124612	batch1_63	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124613	batch1_101	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124614	batch1_28	Beads \| timepoint:4 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124615	batch1_116	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124616	batch1_31	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124617	batch1_117	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124618	batch1_45	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124619	batch1_51	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124620	batch1_65	Beads \| timepoint:6 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124621	batch1_62	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124622	batch1_108	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124623	batch1_123	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124624	batch1_59	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124625	batch1_18	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124626	batch1_9	Beads \| timepoint:8 \| il2_conc:20 \| carrier_conc:n/a \| abs_surface_density:n/a
SA124633	batch2_108	Buffer_control_last \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124634	batch1_130	Buffer_control_last \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124627	batch1_48	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124628	batch1_96	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124629	batch2_1	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124630	batch2_96	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124631	batch2_48	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124632	batch1_1	Buffer_control \| timepoint:n/a \| il2_conc:n/a \| carrier_conc:n/a \| abs_surface_density:n/a
SA124635	batch1_128	DMS \| timepoint:11 \| il2_conc:10 \| carrier_conc:1500 \| abs_surface_density:80
SA124636	batch1_16	DMS \| timepoint:11 \| il2_conc:10 \| carrier_conc:2500 \| abs_surface_density:100
SA124637	batch1_89	DMS \| timepoint:11 \| il2_conc:10 \| carrier_conc:2500 \| abs_surface_density:60
SA124638	batch1_5	DMS \| timepoint:11 \| il2_conc:10 \| carrier_conc:500 \| abs_surface_density:100
SA124639	batch1_40	DMS \| timepoint:11 \| il2_conc:10 \| carrier_conc:500 \| abs_surface_density:60
SA124640	batch1_70	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:1500 \| abs_surface_density:100
SA124641	batch1_34	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:1500 \| abs_surface_density:60
SA124642	batch1_6	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:20 \| abs_surface_density:20
SA124643	batch1_79	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:20 \| abs_surface_density:20
SA124644	batch1_106	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:2500 \| abs_surface_density:80
SA124645	batch1_37	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:2500 \| abs_surface_density:80
SA124646	batch1_110	DMS \| timepoint:11 \| il2_conc:20 \| carrier_conc:500 \| abs_surface_density:80
SA124647	batch2_21	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:1282
SA124648	batch2_20	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:1729
SA124649	batch1_82	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:80
SA124650	batch1_112	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:80
SA124651	batch1_21	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:100
SA124652	batch2_71	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:1282
SA124653	batch2_61	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:1729
SA124654	batch1_90	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:60
SA124655	batch1_27	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:100
SA124656	batch2_103	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:1282
SA124657	batch2_15	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:1729
SA124658	batch1_33	DMS \| timepoint:11 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:60
SA124659	batch2_46	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:1000 \| abs_surface_density:1282
SA124660	batch2_7	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:1000 \| abs_surface_density:1729
SA124661	batch2_14	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:2000 \| abs_surface_density:1282
SA124662	batch2_91	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:2000 \| abs_surface_density:1729
SA124663	batch2_32	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:3000 \| abs_surface_density:1282
SA124664	batch2_72	DMS \| timepoint:11 \| il2_conc:35 \| carrier_conc:3000 \| abs_surface_density:1729
SA124665	batch2_77	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:1500 \| abs_surface_density:1282
SA124666	batch2_83	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:1500 \| abs_surface_density:1729
SA124667	batch2_40	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:2500 \| abs_surface_density:1282
SA124668	batch2_4	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:2500 \| abs_surface_density:1729
SA124669	batch2_73	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:3500 \| abs_surface_density:1282
SA124670	batch2_98	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:3500 \| abs_surface_density:1729
SA124671	batch2_54	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:500 \| abs_surface_density:1282
SA124672	batch2_60	DMS \| timepoint:11 \| il2_conc:40 \| carrier_conc:500 \| abs_surface_density:1729
SA124673	batch1_52	DMS \| timepoint:14 \| il2_conc:10 \| carrier_conc:1500 \| abs_surface_density:80
SA124674	batch1_15	DMS \| timepoint:14 \| il2_conc:10 \| carrier_conc:2500 \| abs_surface_density:100
SA124675	batch1_10	DMS \| timepoint:14 \| il2_conc:10 \| carrier_conc:2500 \| abs_surface_density:60
SA124676	batch1_74	DMS \| timepoint:14 \| il2_conc:10 \| carrier_conc:500 \| abs_surface_density:100
SA124677	batch1_107	DMS \| timepoint:14 \| il2_conc:10 \| carrier_conc:500 \| abs_surface_density:60
SA124678	batch1_55	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:1500 \| abs_surface_density:100
SA124679	batch1_109	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:1500 \| abs_surface_density:60
SA124680	batch1_7	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:20 \| abs_surface_density:20
SA124681	batch1_84	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:20 \| abs_surface_density:20
SA124682	batch1_61	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:2500 \| abs_surface_density:80
SA124683	batch1_124	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:2500 \| abs_surface_density:80
SA124684	batch1_88	DMS \| timepoint:14 \| il2_conc:20 \| carrier_conc:500 \| abs_surface_density:80
SA124685	batch2_50	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:1282
SA124686	batch2_84	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:1729
SA124687	batch1_129	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:80
SA124688	batch1_43	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:1500 \| abs_surface_density:80
SA124689	batch1_64	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:100
SA124690	batch2_59	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:1282
SA124691	batch2_37	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:1729
SA124692	batch1_49	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:2500 \| abs_surface_density:60
SA124693	batch1_120	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:100
SA124694	batch2_66	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:1282
SA124695	batch2_97	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:1729
SA124696	batch1_91	DMS \| timepoint:14 \| il2_conc:30 \| carrier_conc:500 \| abs_surface_density:60
SA124697	batch2_17	DMS \| timepoint:14 \| il2_conc:35 \| carrier_conc:1000 \| abs_surface_density:1282