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MB Sample ID: SA005556

Local Sample ID:11PHS-95
Subject ID:SU000119
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Genotype Strain:N2
Age Or Age Range:Young adult
Gender:Hermaphrodite
Species Group:Invertebrate

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Combined analysis:

Analysis ID AN000165 AN000166
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units

MS:

MS ID:MS000134
Analysis ID:AN000165
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm.
Ion Mode:POSITIVE
Capillary Temperature:325 ?C
Ion Source Temperature:350 ?C
Ion Spray Voltage:3.5 kV
Resolution Setting:70000
  
MS ID:MS000135
Analysis ID:AN000166
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm.
Ion Mode:NEGATIVE
Capillary Temperature:325 ?C
Ion Source Temperature:350 ?C
Ion Spray Voltage:3.5 kV
Resolution Setting:70000
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