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MB Sample ID: SA052780
| Local Sample ID: | Control_4.3 |
| Subject ID: | SU000935 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Daoy (ATCC® HTB-186™) |
| Subject Comments: | cancer cell line |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001460 | AN001461 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) | Waters Acquity BEH Amide (50 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 QTOF | Waters Synapt G2 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
MS:
| MS ID: | MS001348 |
| Analysis ID: | AN001460 |
| Instrument Name: | Waters Synapt G2 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The raw data was converted to NetCDF files using the software DataBridge (Masslynx version 4.1, Waters). Peak detection and retention time alignment was performed using the R based software XCMS (Smith et al. 2006). The centWave function was used for peak detection and the function parameters were set as follows; the maximal deviation in m/z between scans was set to 8 ppm, the maximal and minimal peakwidth was set to 5 and 25 s respectively and the signal to noise ratio cutoff was set to 10. Retention time correction was performed using the “obiwarp” function. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 500°C |
| Capillary Voltage: | 1 kV |
| Collision Energy: | 20- 45 eV collision ramp, MSE acquistion |
| Source Temperature: | 120°C |
| Dataformat: | NetCDF |
| Scan Range Moverz: | m/z 50-800 |
| MS ID: | MS001349 |
| Analysis ID: | AN001461 |
| Instrument Name: | Waters Synapt G2 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The raw data was converted to NetCDF files using the software DataBridge (Masslynx version 4.1, Waters). Peak detection and retention time alignment was performed using the R based software XCMS (Smith et al. 2006). The centWave function was used for peak detection and the function parameters were set as follows; the maximal deviation in m/z between scans was set to 8 ppm, the maximal and minimal peakwidth was set to 5 and 25 s respectively and the signal to noise ratio cutoff was set to 10. Retention time correction was performed using the “obiwarp” function. |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 450°C |
| Capillary Voltage: | 2 kV |
| Collision Energy: | 20- 45 eV collision ramp, MSE acquistion |
| Source Temperature: | 120°C |
| Dataformat: | NetCDF |
| Scan Range Moverz: | m/z 50-800 |
