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MB Sample ID: SA078074
Local Sample ID: | WT3 |
Subject ID: | SU001189 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides thetaiotaomicron VPI-5482 |
Taxonomy ID: | 226186 |
Genotype Strain: | WT (VPI-5482), SPT K.O. (BT_0870) |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | Wild type Bacteroides tethaiotamicron strains and serine palmitoyltransferase (SPT) knockouts |
Subject Comments: | Grown in Minimal Media with or without d4-alanine |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001853 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu Nexera X2 |
Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Focus |
Ion Mode | POSITIVE |
Units | Abundances |
MS:
MS ID: | MS001714 |
Analysis ID: | AN001853 |
Instrument Name: | Thermo Q Exactive Focus |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Raw data were processed using TraceFinder 3.1 (Thermo Fisher) and Progenesis QI (Nonlinear dynamics). Retention time, m/z, MS/MS fragmentation were used to confirm the identity of metabolite using authentic reference standards when available (level 1 identifications), in addition to monitoring the product ions produced and the retention time and mass of species belonging to the each lipid class (level 2-3 annotation). |
Ion Mode: | POSITIVE |