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MB Sample ID: SA084816
Local Sample ID: | C2 |
Subject ID: | SU001274 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 12 weeks old |
Weight Or Weight Range: | 25-30 g |
Gender: | Male |
Animal Animal Supplier: | Jax Laboratories |
Animal Housing: | Conventional |
Animal Light Cycle: | regular 12h light /dark cycles |
Animal Feed: | ad libitum normal chow diet |
Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002009 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Ekspert MicroLC 200 system |
Column | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Peak Area |
MS:
MS ID: | MS001862 |
Analysis ID: | AN002009 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX). C18SPE cartridges were purchased from Biotage. All solvents were of HPLC or LC-MS/MS grade and were acquired from Sigma-Aldrich, Fisher Scientific, or VWR International. |
Ion Mode: | NEGATIVE |