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MB Sample ID: SA092015

Local Sample ID:CB_25
Subject ID:SU001334
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Cell Strain Details:INS-1 832/13 cell line

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Combined analysis:

Analysis ID AN002103
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Relative Intensity

MS:

MS ID:MS001954
Analysis ID:AN002103
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:We used DDA mode to acquire the MS and MS/MS data. Progenesis QI was used for peak picking, alignment, and normalization.
Ion Mode:POSITIVE
Capillary Temperature:275 °C
Capillary Voltage:3.75 KV
Collision Energy:10-35, ramp
Collision Gas:N2
Dry Gas Flow:45
Dry Gas Temp:325°C
Fragmentation Method:CID
Ionization:ES+
Mass Accuracy:5ppm
Dataformat:Profile
Desolvation Gas Flow:45
Desolvation Temperature:325°C
Resolution Setting:120000
Scan Range Moverz:70-1050 m/z
Scanning Range:70-1050 m/z
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