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MB Sample ID: SA097566
Local Sample ID: | SD5b |
Subject ID: | SU001413 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002233 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish UHPLC |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | peak area |
MS:
MS ID: | MS002079 |
Analysis ID: | AN002233 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | LC-MS/MS raw data were imported, processed, normalized and reviewed using Progenesis QI v.2.1 (Non-linear Dynamics, Newcastle, UK). All FMS, DDA and PRM sample runs were chromatographically aligned against a QC reference run. Following peak picking, unique spectral features (retention time and m/z pairs) were grouped based on adducts and isotopes, and individual features or metabolites were normalized to all features. Further filtering was carried out by removing features or metabolites that had >30% coefficient of variance. |
Ion Mode: | NEGATIVE |