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MB Sample ID: SA099252
| Local Sample ID: | Sample_15137 |
| Subject ID: | SU001439 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 21-56 |
| Gender: | Male and female |
| Human Inclusion Criteria: | Cystic Fibrosis, adults |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002272 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Quattro Premier XE |
| Ion Mode | POSITIVE |
| Units | micromolar |
MS:
| MS ID: | MS002116 |
| Analysis ID: | AN002272 |
| Instrument Name: | Waters Quattro Premier XE |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The retention times (RT) and MS/MS parameters for reach antibiotic were determined using the QuanOpt function in the Waters Masslynx software. Peaks were automatically picked and filtered in QuanLynx. Downstream filtering was conducted in R. Data were imported into R and the following criteria for filtering were applied: minimum peak area under the curve of 20 and signal to noise ratio of 10. Because several of the antibiotics had high carry-over rates (including ciprofloxacin, levofloxacin, and trimethoprim), sample peaks were also filtered out when the AUC was lower than the wash ran directly before the sample. Scripts can be found at the following github link: https://github.com/tgallagh/LCMS_Antibiotics. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3.3 kV |
| Collision Gas: | Nitrogen |
| Source Temperature: | 125 |
| Desolvation Gas Flow: | 800 L/h |
| Desolvation Temperature: | 400 |
| Acquisition Parameters File: | CF_antibiotics.exp |
| Analysis Protocol File: | CF_antibiotics_quant.mdb |
