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MB Sample ID: SA099417
| Local Sample ID: | MBG_5 |
| Subject ID: | SU001442 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | ~11 weeks (Intervention) |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Laboratories |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002279 | AN002280 | AN002281 | AN002282 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters HILIC (100 x 2.1mm,1.8um) | Waters HILIC (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Abundance | Abundance | Abundance | abundance |
MS:
| MS ID: | MS002123 |
| Analysis ID: | AN002279 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002124 |
| Analysis ID: | AN002280 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002125 |
| Analysis ID: | AN002281 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
| Ion Mode: | POSITIVE |
| MS ID: | MS002126 |
| Analysis ID: | AN002282 |
| Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
| Ion Mode: | NEGATIVE |
