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MB Sample ID: SA136312
Local Sample ID: | 173 |
Subject ID: | SU001684 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | 22 strains from the Collaborative Cross (CC) mouse panel |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002640 |
---|---|
Analysis type | MS |
Chromatography type | Normal phase |
Chromatography system | Waters Acquity UPLC |
Column | Luna Silica (150 x 2mm,3m) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex API 4000 QTrap |
Ion Mode | POSITIVE |
Units | micromolar |
MS:
MS ID: | MS002452 |
Analysis ID: | AN002640 |
Instrument Name: | ABI Sciex API 4000 QTrap |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Analytes were monitored using electrospray ionization in positive-ion mode with multiple reaction monitoring (MRM) of precursor and characteristic production transitions as shown in MS_protocol.pdf. The parameters for the ion monitoring were as follows: spray voltage, 4.5 kV; curtain gas, 15; GS1, 60; GS2, 50; CAD gas, medium; Nitrogen (99.95% purity) was used as the source and collision gas. Integration and quantification of values was done using Analyst 1.6.2 software (AB SCIEX, Singapore). Standard linearity was calculated using linear regression model. Please see LC_protocol.pdf and MS_protocol.pdf for additional details. |
Ion Mode: | POSITIVE |
Analysis Protocol File: | phoebeyam_LC_protocol.pdf |