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MB Sample ID: SA152156
Local Sample ID: | mv08618_47.mzXML |
Subject ID: | SU001739 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Pooled |
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Combined analysis:
Analysis ID | AN002713 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6550 |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | POSITIVE |
Units | peak area |
MS:
MS ID: | MS002510 |
Analysis ID: | AN002713 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Full scan MS data from 50 to 1700 m/z with a scan frequency of 6 Hz was collected both in positive (ESI+) and negative (ESI-) electrospray ionization modes. The following electrospray ionization parameters were used: gas temperature, 200 °C; drying gas, 14 l min-1; nebulizer, 60 psi; sheath gas temperature, 350 °C; sheath gas flow, 11 l min-1. Urine samples were randomly injected in the chromatographic system in order to avoid intra-batch variability, as well as to enhance quality and reproducibility. QC sample was analyzed every 7 injections to monitor and correct changes in the instrument response. QC sample was also repeatedly analysed under auto MS/MS and All-ion (MSE) fragmentation modes to provide useful information of fragment ions for identification purposes. Sample stability and analytical drift were investigated through IS intensities. Blank analysis was performed at the end of the sequence and used to identify artefacts from sampling, preparation of samples and analysis. |
Ion Mode: | POSITIVE |