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MB Sample ID: SA154480
Local Sample ID: | GOLPH3_OE_2 |
Subject ID: | SU001751 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002732 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Shimadzu Prominence UFPLC xr system |
Column | HILIC Kinetex (50 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Ion Mode | POSITIVE |
Units | intensities |
MS:
MS ID: | MS002529 |
Analysis ID: | AN002732 |
Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data were acquired in full-scan mode at high resolution on a hybrid Orbitrap Elite (Thermo Fisher Scientific, Bremen, Germany). The system was operated at 240,000 resolution (m/z 400) with an AGC set at 1.0E6 and one microscan set at 10-ms maximum injection time. The heated electrospray source HESI II was operated in positive mode at a temperature of 90 C and a source voltage at 4.0KV. Sheath gas and auxiliary gas were set at 20 and 5 arbitrary units, respectively, while the transfer capillary temperature was set to 275 °C. Mass spectrometry data were acquired with LTQ Tuneplus2.7SP2 and treated with Xcalibur 4.0QF2 (Thermo Fisher Scientific). Lipid identification was carried out with Lipid Data Analyzer II (LDA v. 2.6.3, IGB-TUG Graz University) (Hartler et al., 2011). The LDA algorithm identifies peaks by their respective retention time, m/z and intensity. Care was taken to calibrate the instrument regularly to ensure a mass accuracy consistently lower than 3 ppm thereby leaving only few theoretical possibilities for elemental assignment. |
Ion Mode: | POSITIVE |