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MB Sample ID: SA164950
Local Sample ID: | S5R2 |
Subject ID: | SU001853 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002883 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | arbitray unit |
MS:
MS ID: | MS002676 |
Analysis ID: | AN002883 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The MS acquisition parameters were as follows: spray voltage, 3.5 kV; capillary temperature, 320 °C; sheath gas flow rate, 45; auxiliary gas flow rate, 25; heater temperature 350 °C; AGC, 3×106; maximum injection time, 200 ms; mass scan range, 50–750; full MS resolution, 70,000 FWHM at m/z 200; spectrum data type, profile. The raw LC-HRMS files for the same study were processed in one batch using the label-free differential analysis software (SIEVE 2.2, Thermo Scientific), in which the ChromAlign algorithm was used. The key parameters for the feature extraction were as follows: signal to background noise,>3; Mzstep accuracy, 10 ppm, minimum peak intensity 200,000; minimum peak scan points, 5; minimum isotopes,1. |
Ion Mode: | POSITIVE |