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MB Sample ID: SA168239
Local Sample ID: | GATp2GLU-3-pos-PRM |
Subject ID: | SU001888 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Genotype Strain: | Col-0 |
Age Or Age Range: | 10 day old seedlings |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002935 | AN002936 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | umol g FW-1 | umol g FW-1 |
MS:
MS ID: | MS002726 |
Analysis ID: | AN002935 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The following heated electrospray ionization (HESI) conditions were optimized for the analysis of amino and organic acids: spray voltage, 3.9 kV (ESI+), 3.5 kV (ESI-); capillary temperature, 250 °C; probe heater temperature, 450 °C; sheath gas, 30 arbitrary units; auxiliary gas, 8 arbitrary units; and S-Lens RF level, 60%. Injections of 5 μl were used with a flow rate of 0.3 mL min-1. Compounds were detected and monitored using targeted MS/MS, spectra were collected at 17,500 resolution, AGC target 1e6, maximum IT 65 ms, isolation window of 1 m/z, normalized collision energy of 30, intensity threshold of 1.6e5 and 10s dynamic exclusion. |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Targeted_Metabolite_Analysis.pdf |
MS ID: | MS002727 |
Analysis ID: | AN002936 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The following heated electrospray ionization (HESI) conditions were optimized for the analysis of amino and organic acids: spray voltage, 3.9 kV (ESI+), 3.5 kV (ESI-); capillary temperature, 250 °C; probe heater temperature, 450 °C; sheath gas, 30 arbitrary units; auxiliary gas, 8 arbitrary units; and S-Lens RF level, 60%. Injections of 5 μl were used with a flow rate of 0.3 mL min-1. Compounds were detected and monitored using targeted MS/MS, spectra were collected at 17,500 resolution, AGC target 1e6, maximum IT 65 ms, isolation window of 1 m/z, normalized collision energy of 30, intensity threshold of 1.6e5 and 10s dynamic exclusion. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Targeted_Metabolite_Analysis |