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MB Sample ID: SA184328
| Local Sample ID: | QC_CC_6 |
| Subject ID: | SU002035 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 53 ± 15 |
| Weight Or Weight Range: | 116 ± 25 |
| Gender: | Male and female |
| Human Inclusion Criteria: | (i) body mass index (BMI)>35kg/m2 plus GFR 30–60ml/min and proteinuria >1 g/24h or GFR>60ml/min and proteinuria >2.5 g/24h despite receiving maximally tolerated doses of renin-angiotensin-aldosterone system (RAAS) blocker and (ii) BMI>40kg/m2 with a GFR>30ml/min and proteinuria >0.5 g/24h despite receiving maximally tolerated doses of RAAS blocker. For the renal function criterion, eGFR was used. The follow-up time was 24 months. |
| Human Exclusion Criteria: | 1) Patients who had participated or were participating in another clinical trial or had taken an experimental drug in the last 28 days. 2) Patients with renal transplantation and/or chronic replacement therapy (hemodialysis and/or peritoneal dialysis). 3) Subjects with poorly controlled blood pressure (SBP > 170 mmHg or DBP > 110 mmHg). 4) Patients with a history of cardiovascular events in the past six months. 5) Patients treated with immunosuppressants. 6) Subjects with a history of renovascular disease, autoimmune diseases, cancer, drug use, or obstructive uropathy. 6) Patients who did not sign the informed consent. 7) Patients who were pregnant or lactating. eGFR was used to establish the renal function criteria. Patients were monitored for 24 months. |
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Combined analysis:
| Analysis ID | AN003187 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | EI |
| MS instrument type | CE-TOF-MS |
| MS instrument name | Agilent 6224 TOF |
| Ion Mode | POSITIVE |
| Units | Area |
MS:
| MS ID: | MS002965 |
| Analysis ID: | AN003187 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | CE-TOF-MS |
| MS Type: | EI |
| MS Comments: | Ions in gaseous state were directed to the TOF using two different voltages for the fragmentor (125 V and 200 V) and voltages of 65 V and 750 V for the skimmer and the octopole, respectively. Mass range was set from 70 to 1050 Da at a scanning rate of 1.02 scans per second. Data was collected in ESI positive ion mode. MassHunter Workstation version B.06.01 (Agilent Technologies) was used to monitor CE-MS analysis. Voltage of 125 V in the fragmentor was applied to ordinary samples and QC control samples. Data acquired at 125 V, equivalent to MS1, provided information about adducts, isotopes, multimers and fragments ions with low intensity. Voltage of 200 V in the fragmentor was applied to four QC (covering the four types of QC prepared). Data acquired at 200 V is similar to data obtained in 10 eV MS/MS, although has been previously reported that some fragment ions could differ. An equilibration QC was prepared to adapt and prepare the equipment for the conditions of the samples to be analysed. Equilibration QC was prepared as a pool of the four types of QC used to cover completely the biological diversity of the samples. Equilibration QC was analysed before samples. QC samples were disposed throughout the instrumental analysis. |
| Ion Mode: | POSITIVE |
