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MB Sample ID: SA205779
| Local Sample ID: | shchrebp HG-4 |
| Subject ID: | SU002230 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003511 | AN003512 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Fusion Orbitrap | Thermo Fusion Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AUC/ngDNA | AUC/ngDNA |
MS:
| MS ID: | MS003269 |
| Analysis ID: | AN003511 |
| Instrument Name: | Thermo Fusion Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | A Thermo Fisher Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer with heated electrospray ionization source was operated in data- dependent acquisition mode, in positive ionization mode, with scan ranges of 150-827 and 825-1500 m/z. An Orbitrap resolution of 120,000 (FWHM) was used for MS1 acquisition and spray voltages of 3.6kV and -2.9kV were used for positive and negative ionization modes, respectively. For MS2 and MS3 fragmentation a hybridized HCD/CID approach was used. Each sample was analyzed using 4 x 10 µL injections making use of the two scan ranges, in both ionization modes. Data were analyzed using Thermo Scientific LipidSearch software (version 5.0.63) and R scripts written in house. The intensity of each peak was normalized to total lipid signal and to the internal standard. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003270 |
| Analysis ID: | AN003512 |
| Instrument Name: | Thermo Fusion Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | A Thermo Fisher Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer with heated electrospray ionization source was operated in data- dependent acquisition mode, in negative ionization mode, with scan ranges of 150-827 and 825-1500 m/z. An Orbitrap resolution of 120,000 (FWHM) was used for MS1 acquisition and spray voltages of 3.6kV and -2.9kV were used for positive and negative ionization modes, respectively. For MS2 and MS3 fragmentation a hybridized HCD/CID approach was used. Each sample was analyzed using 4 x 10 µL injections making use of the two scan ranges, in both ionization modes. Data were analyzed using Thermo Scientific LipidSearch software (version 5.0.63) and R scripts written in house. The intensity of each peak was normalized to total lipid signal and to the internal standard. |
| Ion Mode: | NEGATIVE |
