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MB Sample ID: SA217813
| Local Sample ID: | A03-02_59_1_1702 |
| Subject ID: | SU002359 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | Sigma-Aldrich (St. Louis, MO) |
| Cell Strain Details: | Hep3B |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003715 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UHPLC-QTOF-MS |
| Column | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Trapped Ion Mobility Q-TOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
MS:
| MS ID: | MS003464 |
| Analysis ID: | AN003715 |
| Instrument Name: | Bruker timsTOF |
| Instrument Type: | Trapped Ion Mobility Q-TOF |
| MS Type: | ESI |
| MS Comments: | For MS2 acquisition in metabolomics analysis, the collision energy was fluctuated between 100-250% of 20 eV and end plate offset of 500V. The acquisition was in two sections: auto MS scan for the calibrant sodium formate in 0-0.3 min, and auto MS/MS for fragmentation, in 0.3 to 30 min. Positive mode at 12 Hz was performed in both acquisition sections . The scan range was 20 to 1300 m/z, the precursor ion’s width of ±0.5, the precursors number of 3 , the cycle time of 0.5 s econds, and the threshold of 400 counts. After three spectra, active exclusion was performed and released after 0.2 min. A timsTOF (Bruker, Bremen, Germany) with an Apollo II electrospray ionization (ESI) source was utilized for the MS analysis with the following parameters: the nebulizer pressure was 2.2 bar, the drying gas flow rate was 10 L/min, the drying tempera-ture was 220°C, and the capillary voltage was 4500 V. In the first 0.3 min of each LC-MS/MS run, the external cali-brant, sodium formate, was injected. Mass calibration was done prior to analysis ac-cording to the manufacturer’s recommendations using external mass calibration (10 mM sodium formate calibrant solution). The performance of the column and the mass spectrometer was tested using a test mixture of (TRX-2101/RT-28-calibrants for Bruker T-ReX LC-QTOF solution from Nova Medical Testing Inc.) to check the performance of reversed-phase liquid chromatography (RPLC) separation and perform multipoint re-tention time calibration, and (TRX-3112-R/MS Certified Human serum for Bruker T-ReX LC-QTOF solution from Nova Medical Testing Inc.) to check the performance of sample preparation protocols as well as LC-MS instruments. This product is prepared from pooled human blood. |
| Ion Mode: | POSITIVE |
