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MB Sample ID: SA218940
Local Sample ID: | IL04_1_36_CETOF |
Subject ID: | SU002371 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens + Leishmania donovani |
Taxonomy ID: | 9606 and 5661 |
Genotype Strain: | Human: PBMCs from 7 human donors. L. donovani: MHOM/IN/82/Patra1 and MHOM/ET/1967/Hu3 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003734 | AN003735 | AN003736 | AN003737 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | CE | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 7100 CE | Agilent 1200 | Agilent 1200 | Agilent 1290 Infinity |
Column | bare-fused silica capillary (96 cm,50um),Agilent Technologies | Agilent Zorbax Extend C18 RRHT (50 x 2.1mm,1.8 um) | Agilent Zorbax Extend C18 RRHT (50 x 2.1mm,1.8 um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | TOF | QTOF | QTOF | Triple quadrupole |
MS instrument name | Agilent 6224 TOF | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 6460 QQQ |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | Abundances | Abundances | Abundances | Normalized abundances |
MS:
MS ID: | MS003482 |
Analysis ID: | AN003734 |
Instrument Name: | Agilent 6224 TOF |
Instrument Type: | TOF |
MS Type: | ESI |
MS Comments: | mass range mz 68 to 1000 mass correction mz 121.0509 and 922.0098 mass calibrators Acq mode full scan AMH Profinder B.08.00 for feature deconvolution CMM Batch Search and in house library of standards with RMT |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 V |
Dry Gas Flow: | 10 L/min |
Dry Gas Temp: | 200 ºC |
Fragment Voltage: | 125 V |
Nebulizer: | 10 psi |
Octpole Voltage: | 750 V |
MS ID: | MS003483 |
Analysis ID: | AN003735 |
Instrument Name: | Agilent 6545 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | full MS: mass range mz 50 to 3000 mass correction mz 121.0509 and 922.0098 mass calibrators iterative MSMS of pooled samples isolation width = 1.3 Da CE with slope and offset of 3.8 and 4.6, respectively AMH Profinder B.08.00 for feature deconvolution CMM Batch Search and in house library of standards with RMT AMH LipidAnnotator for in silico anotation of MSMS spectra |
Ion Mode: | POSITIVE |
Capillary Voltage: | 4000 V |
Dry Gas Flow: | 12 L/min |
Dry Gas Temp: | 250 ºC |
Fragment Voltage: | 125 V |
Nebulizer: | 52 psi |
Octpole Voltage: | 750 V |
MS ID: | MS003484 |
Analysis ID: | AN003736 |
Instrument Name: | Agilent 6545 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | full MS: mass range mz 50 to 3000 mass correction mz 121.0509 and 922.0098 mass calibrators iterative MSMS of pooled samples isolation width = 1.3 Da CE with slope and offset of 3.8 and 4.6, respectively AMH Profinder B.08.00 for feature deconvolution CMM Batch Search and in house library of standards with RMT AMH LipidAnnotator for in silico anotation of MSMS spectra |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 4000 V |
Dry Gas Flow: | 12 L/min |
Dry Gas Temp: | 250 ºC |
Fragment Voltage: | 125 V |
Nebulizer: | 52 psi |
Octpole Voltage: | 750 V |
MS ID: | MS003485 |
Analysis ID: | AN003737 |
Instrument Name: | Agilent 6460 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | MRM isolation width narrow 1.3 Da AMH Quantitative Analysis (Quant My Way) for targeted data integration cycle time = 650 ms MRM repeats = 3 nozzle voltage = 500 V |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 2000 V |
Dry Gas Flow: | 12 L/min |
Dry Gas Temp: | 325 ºC |
Nebulizer: | 45 psi |