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MB Sample ID: SA232066
| Local Sample ID: | 16 |
| Subject ID: | SU002421 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Strain Details: | The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. |
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Combined analysis:
| Analysis ID | AN003810 | AN003811 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | ESI | ESI |
| MS instrument type | hybrid triple quadrupole/linear ion trap | hybrid triple quadrupole/linear ion trap |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol lipid per mg protein | nmol lipid per mg protein |
MS:
| MS ID: | MS003552 |
| Analysis ID: | AN003810 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | hybrid triple quadrupole/linear ion trap |
| MS Type: | ESI |
| MS Comments: | SM, CE, CER, DCER, HCER, LCER were measured in positive ion mode with a precursor scan of 184.1, 369.4, 264.4, 266.4, 264.4 and 264.4 respectively. TAG, DAG and MAG were measured in positive ion mode with a neutral loss scan for one of the fatty acyl moieties. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003553 |
| Analysis ID: | AN003811 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | hybrid triple quadrupole/linear ion trap |
| MS Type: | ESI |
| MS Comments: | PC, LPC, PE, LPE, PG, PI and PS were measured in negative ion mode by fatty acyl fragment ions. Lipid quantification was performed by scheduled multiple reactions monitoring (MRM), the transitions being based on the neutral losses or the typical product ions as described above. The instrument parameters were as follows: Curtain Gas = 35 psi; Collision Gas = 8 a.u. (medium); IonSpray Voltage = 5500 V and −4,500 V; Temperature = 550°C; Ion Source Gas 1 = 50 psi; Ion Source Gas 2 = 60 psi; Declustering Potential = 60 V and −80 V; Entrance Potential = 10 V and −10 V; Collision Cell Exit Potential = 15 V and −15 V. |
| Ion Mode: | NEGATIVE |
