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MB Sample ID: SA235362
| Local Sample ID: | Cal5_B |
| Subject ID: | SU002434 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 12-24 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson |
| Animal Light Cycle: | 12L/12D |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003829 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid |
| Ion Mode | UNSPECIFIED |
| Units | ug/mL |
MS:
| MS ID: | MS003571 |
| Analysis ID: | AN003829 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Raw data files were brought into Skyline software. Targeted peak detection was done based on the parent mass. Mass analyzer set to Orbitrap and resolution power set to 120,000 resolution. Then all raw files and unknown samples were imported to Skyline. Calibration curves were generated by Linear regression fit. Targeted precursor MZ and MZ of analytes was used to track and quantification of the metabolite. Peak areas for analytes in samples were used for quantification based in the generated calibration curves. |
| Ion Mode: | UNSPECIFIED |
