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MB Sample ID: SA237004
| Local Sample ID: | S14_B.cells_tfgKO_1E10 |
| Subject ID: | SU002449 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003854 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | SCIEX QTRAP 6500 |
| Ion Mode | POSITIVE |
| Units | counts per second (cps) |
MS:
| MS ID: | MS003595 |
| Analysis ID: | AN003854 |
| Instrument Name: | SCIEX QTRAP 6500 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Mass spectrometric analysis was performed using the QTRAP 6500 (SCIEX) operated by Analyst 1.6.3. The following instrument dependent settings were used: curtain gas, 20 psi; CAD gas, medium; and interface heater temperature, 100°C. PC analysis was performed in the positive ion mode by scanning for precursors of m/z 184 at a collision energy of 35 eV. PE, PS, PG, PI, and PA measurements were performed in the positive ion mode by scanning for neutral losses of 141, 185, 189, 277, and 115 D at CE of 25 eV. The value for the declustering potential was 100 V (Özbalci et al. 2013, Methods Mol Biol 1033:3-20). Scanning was performed in a mass range of m/z 650–900 D and at a scan rate of 200 D/s. 61 MCA spectra were accumulated. Mass spectra were processed by the LipidView Software Version 1.2 (SCIEX) for identification and quantification of glycerophospholipids. Endogenous glycerophospolipids were quantified by referring their peak areas to those of the internal standards. |
| Ion Mode: | POSITIVE |
