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MB Sample ID: SA245934
Local Sample ID: | 831_SFG_WM |
Subject ID: | SU002546 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004007 | AN004008 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | pmoles/mg protein | pmoles/mg protein |
MS:
MS ID: | MS003754 |
Analysis ID: | AN004007 |
Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data was acquired in full scan/data-dependent MS2 (full scan resolution 60,000 FWHM, scan range 220–1600 m/z) in both positive and negative ionization modes. The ten most abundant ions in each cycle were subjected to MS2, with an isolation window of 1.4 m/z, collision energy 30 eV, resolution 17,500 FWHM, maximum integration time 110 ms and dynamic exclusion window 10 s. An exclusion list of background ions was used based on a solvent blank. An inclusion list of the [M+H]+ and [M-H]- ions was used for all internal standards. LipidSearch software v4.2 (Thermo Fisher) was used for lipid annotation, chromatogram alignment, and peak integration from extracted ion chromatograms. Lipid annotation was based on precursor and product ions in both positive and negative ion mode. Individual lipids were expressed as ratios to an internal standard specific for each lipid class, then multiplied by the amount of internal standard added to produce a molar amount of each lipid per sample, which was normalised to protein amount in each sample. |
Ion Mode: | POSITIVE |
MS ID: | MS003755 |
Analysis ID: | AN004008 |
Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data was acquired in full scan/data-dependent MS2 (full scan resolution 60,000 FWHM, scan range 220–1600 m/z) in both positive and negative ionization modes. The ten most abundant ions in each cycle were subjected to MS2, with an isolation window of 1.4 m/z, collision energy 30 eV, resolution 17,500 FWHM, maximum integration time 110 ms and dynamic exclusion window 10 s. An exclusion list of background ions was used based on a solvent blank. An inclusion list of the [M+H]+ and [M-H]- ions was used for all internal standards. LipidSearch software v4.2 (Thermo Fisher) was used for lipid annotation, chromatogram alignment, and peak integration from extracted ion chromatograms. Lipid annotation was based on precursor and product ions in both positive and negative ion mode. Individual lipids were expressed as ratios to an internal standard specific for each lipid class, then multiplied by the amount of internal standard added to produce a molar amount of each lipid per sample, which was normalised to protein amount in each sample. |
Ion Mode: | NEGATIVE |