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MB Sample ID: SA254230
Local Sample ID: | OC156 |
Subject ID: | SU002621 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004153 | AN004154 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid |
Ion Mode | NEGATIVE | POSITIVE |
Units | chromatographic peak area | chromatographic peak area |
MS:
MS ID: | MS003900 |
Analysis ID: | AN004153 |
Instrument Name: | Thermo Orbitrap ID-X tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data were acquired in the 150-2000 m/z range with a 120,000 mass resolution setting. For MS/MS experiments, the Thermo Scientific Deep AcquireX data acquisition workflow was performed. Stepped normalized collision energy (NCE) of 15,30,45 was used for fragmenting precursor ions in the HCD cell followed by Orbitrap analysis at 30,000 mass resolving power. Precursor ions were also fragmented with CID energy of 40 and were analyzed in the ion trap. Data were processed in Compound Discoverer 3.3. |
Ion Mode: | NEGATIVE |
MS ID: | MS003901 |
Analysis ID: | AN004154 |
Instrument Name: | Thermo Orbitrap ID-X tribrid |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | MS data were acquired in the 150-2000 m/z range with a 120,000 mass resolution setting. The most relevant MS parameters and the chromatographic gradient used are given in supplementary section Table S2 and S3, respectively. For MS/MS experiments, the Thermo Scientific Deep AcquireX data acquisition workflow was performed. Stepped normalized collision energy (NCE) of 15,30,45 was used for fragmenting precursor ions in the HCD cell followed by Orbitrap analysis at 30,000 mass resolving power. Precursor ions were also fragmented with CID energy of 40 and were analyzed in the ion trap. |
Ion Mode: | POSITIVE |