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MB Sample ID: SA258352
| Local Sample ID: | ap1calAP1GR_cytokinins_3 |
| Subject ID: | SU002672 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Genotype Strain: | ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR |
| Age Or Age Range: | Bolting (40-50 days after germination) |
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Combined analysis:
| Analysis ID | AN004236 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
MS:
| MS ID: | MS003983 |
| Analysis ID: | AN004236 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Cytokinins were detected using the positive ion mode. For tZ, tZR, iP, iPR, and the internal control BAP, peaks were identified from an external standard mix composed of 0.1 µg/ml each of BAP (Alfa Aesar A14678), tZ (Sigma Z0876), tZR (Sigma Z3541), iP (Cayman Chemical 17906), and iPR (Cayman chemical 20522) in 5% acetonitrile. For cZ and cZR, peaks were identified based on previously reported precursor m/z and retention time. Using Xcalibur (Thermo Scientific), peak area was quantified for each cytokinin in each sample, normalized against the peak area of BAP (internal control) and sample fresh weight, and then normalized against the average abundance of tZ in WT samples. |
| Ion Mode: | POSITIVE |
