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MB Sample ID: SA264246
Local Sample ID: | 90587016811 |
Subject ID: | SU002764 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004334 |
---|---|
Analysis type | MS |
Chromatography type | Flow induction analysis |
Chromatography system | Waters Acquity UPLC |
Column | No column |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-S |
Ion Mode | POSITIVE |
Units | pmol/mg of tissue |
MS:
MS ID: | MS004081 |
Analysis ID: | AN004334 |
Instrument Name: | Waters Xevo TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Ion ratios of endogenous acyl CoAs to the C-17 CoA internal standard are computed from centroided spectra using a software package NeoLynx (Waters, Milford, MA). The ratios are converted to concentrations using calibrators prepared by spiking tissue homogenates with authentic CoAs (Sigma, St. Louis , MO) having saturated acyl chain lengths C0- C18. Corrections for the heavy isotope effects, mainly 13C, to the adjacent m+2 spectral peaks in a particular chain length cluster are made empirically by referring to the observed spectra for the analytical standards. The values are expressed in pmol/mg. Spectra are acquired in the multichannel acquisition mode monitoring the neutral loss of 507 amu (phosphoadenosine diphosphate) and scanning from m/z 750 to1060. |
Ion Mode: | POSITIVE |