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MB Sample ID: SA272257

Local Sample ID:RF168
Subject ID:SU002812
Subject Type:Mammal
Subject Species:Mus musculus

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Combined analysis:

Analysis ID AN004389
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Ascentis Express C18 (150 x 2.1mm,2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units nM

MS:

MS ID:MS004138
Analysis ID:AN004389
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS data acquisition was performed using a QExactive benchtop orbitrap mass spectrometer equipped with an Ion Max source and a HESI II probe (Thermo Fisher Scientific, San Jose, CA) and was performed at a narrow scan in positive mode (m/z = 600-800) for more specific detection of thyroxine hormones, the resolution was set at 70,000, the AGC target was 5x105, and the max IT was 100 msec. HESI conditions were: Sheath gas flow rate: 40; Aug gas flow rate: 10; Sweet gas flow rate: 0; Spray voltage: 3.5kV (pos), 2.8kV (neg); Capillary temperature: 380ºC; S-lens RF: 60; Aux gas heater temperature: 420 ºC.
Ion Mode:POSITIVE
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