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MB Sample ID: SA308674
| Local Sample ID: | N_AB-C-3 |
| Subject ID: | SU002966 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus aureus/ Acinetobacter baumannii/ Enterococcus faecium/ Pseudomonas aeruginosa |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004675 | AN004676 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 XS QTOF | Waters Synapt G2 XS QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | internsity | intensity |
MS:
| MS ID: | MS004422 |
| Analysis ID: | AN004675 |
| Instrument Name: | Waters Synapt G2 XS QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The UPLC was connected to the electrospray ionization source of the traveling wave ion mobility-mass spectrometer (Waters Synapt XS) and samples were injected at 5 uL. Prior to acquisition of sample data, data was acquired for a mixture of CCS calibrants using direct infusion. Randomized sample queues were analyzed in both positive and negative ionization modes. A pooled mixture of all samples was used as a quality control (QC). Data was collected across the entire 17 min chromatographic method using data-independent MS/MS acquisition. Leucine enkephalin was monitored for post-acquisition lockmass correction. Capillary +3 kV; Sampling Cone 30 V; Sampling Cone 25 V; Source Offset 40 V; Source Temp 150 ºC; Desolvation Temp 400 ºC; Cone gas flow 50 L/h; Desolvation gas flow 650 L/h; Nebulizer gas flow 7 Bar. Mass Range 50-1200 m/z. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004423 |
| Analysis ID: | AN004676 |
| Instrument Name: | Waters Synapt G2 XS QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The UPLC was connected to the electrospray ionization source of the traveling wave ion mobility-mass spectrometer (Waters Synapt XS) and samples were injected at 5 uL. Prior to acquisition of sample data, data was acquired for a mixture of CCS calibrants using direct infusion. Randomized sample queues were analyzed in both positive and negative ionization modes. A pooled mixture of all samples was used as a quality control (QC). Data was collected across the entire 17 min chromatographic method using data-independent MS/MS acquisition. Leucine enkephalin was monitored for post-acquisition lockmass correction. Capillary -2 kV; Sampling Cone 30 V; Sampling Cone 25 V; Source Offset 40 V; Source Temp 150 ºC; Desolvation Temp 400 ºC; Cone gas flow 50 L/h; Desolvation gas flow 650 L/h; Nebulizer gas flow 7 Bar. Mass Range 50-1200 m/z. |
| Ion Mode: | NEGATIVE |
